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Recombinant Rattus Clusterin, His-tagged, T7-tagged

Cat.No. : Clu-108R
Product Overview : The Rat Clusterin was constructed as a recombinant protein with N-terminal fusion of T7-Tag (16AA) and C-terminal fusion of His-Tag (9AA). The Clusterin Rat His-Tagged Fusion Protein, produced inE.coli, is 26.5 kDa protein containing 215 amino acid residues of the APO-J Rat and 25 additional amino acid residues – His-Tag, T7-Tag.
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Cat. No. : Clu-108R
Description : Clusterin also named Apoliprotein J (APO-J) is a 75-80 kD disulfide-linked heterodimeric protein containing about 30% of N-linked carbohydrate rich in sialic acid. The precursor polypeptide chain is cleaved proteolytically to remove the 22-mer secretory signal peptide and subsequently between residues 227/228 to generate and the chains. These are assembled in anti-parallel to give a heterodimeric molecule in which the cysteine-rich centers are linked by five disulfide bridges and are flanked by two predicted coiled-coil -helices and three predicted amphipathic a-helices. It is able to bind and form complexes with numerous partners such as immunoglobulins, lipids, heparin, bacteria, complement components, paraoxonase, beta amyloid, leptin and others.
Source : Escherichia Coli.
Amino acid sequence : MASMTGGQQM GRDPNSSSPF YFWMNGDRID SLLESDRQQS QVLDAMQDSF TRASGIIDTL FQDRFFTHEP QDIHHFSPMG FPHKRPHLLY PKSRLVRSLM PLSHYGPLSF HNMFQPFFDM IHQAQQAMDV QLHSPALQFPDVDFLKEGED DRTVCKEIRH NSTGCLKMKG QCEKCQEILS VDCSTNNPAQ ANLRQELNDS LQVAERLTQQ YNELLHSLQS KMLNTSSLLE QALEHHHHHH
Specificity : The amino acid sequence of the Clusterin Rat recombinant is 100% homologous to the amino acid sequence 146-360 of the Clusterin Rat precursor.
Purification Method : Ni-NTA chromatography.
Purity : Greater than 95% (SDS PAGE analyzed).
Formulation : Sterile filtered and lyophilized from 0.5 mg/ml in 0.01M Tris pH 7.2.
Solubility : Add 0.2 ml of deionized H2O and let the lyophilized pellet dissolve completely.
Applications : Western blotting
Storage : Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. The lyophilized protein remains stable until the expiry date when stored at -20°C.
Gene Name : Clu clusterin [ Rattus norvegicus ]
Synonyms : Clu; Clusterin; CLI; APOJ; SGP2; SGP-2; SP-40; Trpm2; Trpmb; TRPM-2; TRPM2B; RATTRPM2B; CLU; TRPM2; KUB1; AAG4; MGC24903; Dimeric acidic glycoprotein; DAG protein; Testosterone repressed prostate message-2; Sulfated glycoprotein-2; Complement lysis inhibitor;SP-40; MGC24903; Apolipoprotein J
Gene ID : 24854
mRNA Refseq : NM_053021
Protein Refseq : NP_444180
UniProt ID : P05371
Chromosome Location : 15p12
Function : protein binding

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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How does CLU contribute to the regulation of inflammation, and what role does it play in immune responses and autoimmunity? 05/03/2022

CLU has anti-inflammatory properties and modulates immune responses. Its involvement in autoimmunity is linked to its ability to regulate immune cell functions and cytokine production.

How does CLU contribute to the regulation of cell survival and apoptosis, and what are the molecular mechanisms involved? 03/08/2021

CLU plays a role in regulating cell survival and apoptosis by interacting with key cellular components, including Bcl-2 family members and caspases, modulating apoptotic pathways.

How does CLU influence cell adhesion and migration, and what role does it play in tissue remodeling and repair processes? 01/16/2020

CLU modulates cell adhesion and migration, contributing to tissue remodeling and repair. Its interactions with extracellular matrix components influence cellular behaviors in wound healing and tissue homeostasis.

Can you discuss the association between CLU and neurodegenerative disorders, and how does it impact the pathogenesis of conditions like Alzheimer's disease? 11/16/2019

CLU is implicated in neurodegenerative disorders, particularly Alzheimer's disease. Its involvement in processes such as amyloid-beta aggregation and clearance influences disease progression.

Can you elaborate on the molecular interactions of CLU with lipids and its involvement in lipid transport and metabolism? 07/24/2018

CLU interacts with lipids, participating in lipid transport and metabolism. Its role in lipoprotein remodeling and cholesterol homeostasis links it to cardiovascular health.

Customer Reviews (3)

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Reviews
11/30/2021

    Impressed with the quality control. This product is a gem in our lab experiments.

    01/22/2019

      Smooth transactions and swift delivery. Our lab supplies are incomplete without this protein.

      04/17/2018

        Our lab manager introduced us to this product, and we're never going back. Essential for our studies.

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