||Recombinant Human ZO-1 protein expressed inE.coliencodes 334-634 amino acids and has a molecular mass of 61 kDa through SDS-PAGE analysis.
||ZO-1 is a peripheral membrane protein that is associated with tight junctions which are found on the cytoplasmic surface of epitheliual and endothelial cell membrane. ZO-1 has a MW of 225kDa and is found in two isoforms that differ by the presence or absence of an 80 amino acid region known as 'motif-a'.
||Recombinant Human ZO-1 protein encoding 334-634 amino acids expressed inE.coli.
||Recombinant Human ZO-1 protein at 100µg/ml in 50mM Tris-Acetate, pH7.5, 1mM EDTA and 20% Glycerol.
|Application and suggested dilutions:
||•ELISA •Inhibition Assays •Western Blotting
||On SDS-PAGE commassie blue stained gel, the purified recombinant protein shows a band at 61 kDa.
|Storage & stability:
||Store vial at -20°Cto -80°C. When stored at the recommended temperature, this protein is stable for 12 months.