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Recombinant Human Plasminogen

Cat. No.: PLG-66H
Product Overview: Recombinant Human Angiostatin produced inPichia Pastorisis a single, non-glycosylated polypeptide chain having a molecular mass of 29711 Dalton.
Description: RHuAng K1-3 is a proteolytic fragment of plasminogen containing the first three kringle structures. A specific inhibitor of endothelial cell growth and angiogenesis. More active relative to kringles 1-4. rHuAng K1-3 reduces endothelial cell proliferation and acts as a potent inhibitor of angiogenesis and tumor growth. The rHuAng K1-3 is purified by proprietary chromatographic techniques.
Source: Pichia Pastoris.
Physical Appearance: Sterile Filtered lyophilized powder.
Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Formulation: Lyophilized from a (1mg/ml) solution in containing 0.5X PBS.
Solubility: It is recommended to reconstitute the lyophilized rHuAng K1-3 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Biological Activity: Inhibitory activity (ED50=70 nm) relative to Kringles 1-4 (ED50=135 nm). Kringles 1-3 acts as inhibitor of endothelial cell growth and angiogenesis.
Endotoxin: Less than 0.1 ng/µg (IEU/µg) of rHuAng K1-3.
Protein Content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 2.08 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of rHuAng K1-3 as a Reference Standard.
Storage: Lyophilized rHuAng K1-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rHuAng K1-3 should be stored at 4°Cbetween 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Gene Name: PLG plasminogen [ Homo sapiens ]
Synonyms: PLG; plasminogen; DKFZp779M0222; Plasminogen; Plasmin heavy chain A;Activation peptide; Angiostatin; Plasmin heavy chainA, short form; Plasmin light chain B;EC 3.4.21.7; OTTHUMP00000017544
Gene ID: 5340
mRNA Refseq: NM_000301
Protein Refseq: NP_000292
MIM: 173350
UniProt ID: P00747
Chromosome Location: 6q26
Pathway: Complement and coagulation cascades; Neuroactive ligand-receptor interaction; Diabetes pathways; Hemostasis; Signaling by PDGF
Function: calcium ion binding; apolipoprotein binding; peptidase activity; serine-type endopeptidase activity

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