| Cat. No. : |
PLG-66H |
| Description : |
RHuAng K1-3 is a proteolytic fragment of plasminogen containing the first three kringle structures. A specific inhibitor of endothelial cell growth and angiogenesis. More active relative to kringles 1-4. rHuAng K1-3 reduces endothelial cell proliferation and acts as a potent inhibitor of angiogenesis and tumor growth. The rHuAng K1-3 is purified by proprietary chromatographic techniques. |
| Source : |
Pichia Pastoris. |
| Physical Appearance : |
Sterile Filtered lyophilized powder. |
| Purity : |
Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. |
| Formulation : |
Lyophilized from a (1mg/ml) solution in containing 0.5X PBS. |
| Solubility : |
It is recommended to reconstitute the lyophilized rHuAng K1-3 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Biological Activity : |
Inhibitory activity (ED50=70 nm) relative to Kringles 1-4 (ED50=135 nm). Kringles 1-3 acts as inhibitor of endothelial cell growth and angiogenesis. |
| Endotoxin : |
Less than 0.1 ng/µg (IEU/µg) of rHuAng K1-3. |
| Protein Content : |
Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 2.08 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of rHuAng K1-3 as a Reference Standard. |
| Storage : |
Lyophilized rHuAng K1-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rHuAng K1-3 should be stored at 4°Cbetween 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
