||Recombinant Human Insulin promoter factor-1 Protein, produced inE. coli, contains 283 amino acids and has a molecular mass of 30.64 kDa.
||Pancreatic Duodenal Homeobox-1 activates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. Particularly involved in glucose-dependent regulation of insulin gene transcription. binds preferentially the dna motif 5"-[ct]taat[tg]-3". During development, specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. at adult stage, required for maintaining the hormone-producing phenotype of the beta-cell. Mutations in this gene may be involved in several disorders of the pancreas or in diabetes mellitus.
||Sterile Filtered White lyophilized (freeze-dried) powder.
||Lyophilized Powder of liquid in PBS, pH 7.4.
||Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
||It is recommended to reconstitute the lyophilized PDX1 in sterile PBS, 10% glycerol to prepare a stock of 100μg/ml.
||The activity of human PDX-1 is determined by the ability to stimulate NeuroD1 gene expression in WB cells and binding to insulin promoter be EMSA assay.
||Recombinant Human PDX1 although stable at 25°C 1 week, should be stored desiccated below -18°C. Please prevent freeze-thaw cycles.