| Cat. No. : |
REG4-194H |
| Description : |
REG protein was shown to be stimulated during the regeneration of pancreatic islets. Since then, many Reg-related proteins have been identified in humans and other animals. In human, the four REG family genes, i.e., REG 1 alpha, REG 1 beta, REG-related sequence (RS) and HIP/PAP, have so far been isolated. These Reg-related proteins are classified into four subfamilies according to their amino-acid sequences, but they share a similar structure and physiological function. Reg protein is a growth factor for pancreatic beta cells and also suggest that the administration of Reg protein could be used as another therapeutic approach for diabetes mellitus. human REG cDNA which encoded a 166-amino acid protein with a 22-amino acid signal peptide. The amino acid sequence of human REG protein has 68% homology to that of rat Reg protein. Reg I was found to be expressed mainly in pancreatic beta and acinoductular cells as well as gastric fundic enterochromaffin-like (ECL) cells. Reg I production in ECL cells is stimulated by gastrin, as well as by the proinflammatory cytokine, cytokine-induced neutrophil chemoattractant (CINC)-2Beta. |
| Source : |
E.Coli. |
| Sequences Of Amino Acids : |
MKHHHHHHAS HMDIIMRPSC APGWFYHKSN CYGYFRKLRN WSDAELECQS YGNGAHLASI LSLKEASTIA EYISGYQRSQ PIWIGLHDPQ KRQQWQWIDG AMYLYRSWSG KSMGGNKHC AEMSSNNNFLT WSSNECNKRQ HFLCKYRP. |
| Specificity : |
The amino acid sequence of the recombinant Human REG IV is 100% homologous to the amino acid sequence of the Human REG IV without signal sequence. |
| Purification Method : |
Ni-NTA chromatography. |
| Purity : |
>95% (SDS-PAGE analyzed). |
| Formulation : |
Sterile filtered and lyophilized from 0.5 mg/ml in 20 mM Tris , pH 8.0. |
| Reconstitution : |
Add 0.2 ml of deionized H2O and let the lyophilized pellet dissolve completely. |
| Stability/Shelf Life : |
The lyophilized protein remains stable until the expiry date when stored at -20°C. |
| Applications : |
Western blotting, ELISA. |
| Storage : |
Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. |
