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Recombinant Human Noggin

Cat.No. : NOG-454H
Product Overview : Recombinat human Noggin was expressed in modifiedhuman 293 cells.
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Cat. No. : NOG-454H
Description : Noggin is a secreted glycoprotein predominantly expressed by the dorsal mesoderm during embryogenesis. Noggin has been shown to bind with high affinity to bone morphogenetic protein-4 (BMP-4) and with lower affinity to BMP-2 and BMP-7. Noggin specifically inhibits the action of BMP's by preventing interaction with their receptors. It has been shown that noggin maintains hES in an undifferentiated state in culture. Additionally, noggin in combination with FGF2 can maintain undifferentiated hES growth for substantial culture periods while retaining hES karyotype and pluripotency. The role of noggin in the maintenance of undifferentiated hES suggests it is potentially a valuable component of animal free hES cultures allowing hES to be applicable for therapeutic applications. Human noggin is a 205 amino acid glycoprotein and is secreted as a covalently linked homodimer of approximately 30 kDa with a similar structure to BMP-7.
Source : human 293 cells
Amino Acid Sequence : QHYLHIRPAPS DNLPLVDLIEHPDPIFDPK EKDLNETLLRSLLGGHYD PGFM ATSP P ED RPG GGGGA AGG AEDLAELDQLLRQRP SGA MPS EIKGLE FSEGLA Q GKKQRLSKKLRRKLQMWL WSQTFCPVLYAWNDLGS RFWPR YVK V GSCFSKRS CSVPEGMVCKPSKSVHL TVL RWR CQRR GGQ RCGW IPIQY PIISECKCSC.
Molecular Mass : Noggin migrates as a band between 25 and 30 kDa in SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares with the unmodified Noggin that has a predicted molecular mass of 23.0kDa.
PI : Noggin separates into a number of isoforms with a pI between 4.2 and 5.3 in 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified Noggin that has a predicted pI of 9.1.
% Carbohydrate : Noggin consists of 5-25% carbohydrate by weight.
Glycosylation : Noggin has N-linked oligosaccharides.
Purity : >95% as determined by SDS-PAGE and visualized by silver stain.
Formulation : When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
Reconstitution : It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
Storage : Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C.
Gene Name : NOG noggin [ Homo sapiens ]
Synonyms : noggin; SYM1; SYNS1; NOG; symphalangism 1 (proximal); synostoses (multiple) syndrome 1
Gene ID : 9241
mRNA Refseq : NM_005450
Protein Refseq : NP_005441
MIM : 602991
UniProt ID : Q13253
Chromosome Location : 17q21-q22
Pathway : TGF-beta signaling pathway; Signaling by BMP
Function : protein homodimerization activity; cytokine binding

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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How does NOG (Noggin) modulate bone morphogenetic protein (BMP) signaling in development and tissue repair? 10/25/2022

NOG inhibits BMP signaling, critical for tissue development and repair processes.

What are the mechanisms by which NOG modulates embryonic patterning and organogenesis? 09/05/2021

NOG modulates embryonic patterning and organogenesis, guiding proper organ development.

How do alterations in NOG expression or function affect the development of congenital anomalies? 02/08/2020

Changes in NOG expression or function can lead to congenital anomalies due to disrupted developmental processes.

How does NOG contribute to the regulation of stem cell differentiation? 07/11/2019

NOG regulates stem cell differentiation, influencing their development into various tissue types.

What role does NOG play in skeletal development and the regulation of bone growth? 02/11/2019

NOG plays a key role in skeletal development by regulating bone growth and differentiation.

How does NOG influence neural development and neurogenesis? 02/03/2019

NOG influences neural development, impacting the formation and specialization of neural cells.

What is the impact of NOG in joint and cartilage health, particularly in arthritis? 01/07/2018

NOG is important for joint and cartilage health, potentially impacting arthritis progression.

Customer Reviews (3)

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Reviews
07/15/2022

    Efficient peptide synthesis, accelerates our peptide research projects.

    04/30/2018

      Fast and reliable protein expression, aids our experiments significantly.

      01/25/2018

        Dependable antibody validation, crucial for our immunoprecipitation studies.

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