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PGK1Recombinant Human Phosphoglycerate Kinase 1
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| Cat. No. : | PGK1-187H |
| Product Overview : | Recombinant PGK1 protein was expressed inE.coliand purified by using conventional chromatography techniques. MW = 46.8 kDa (437aa) |
| Description : | Phosphoglycerate kinase 1 (PGK1) is an X-linkedenzymethat plays a key role in the glycolytic pathway. The gene encoding the erythrocyte enzyme PGK1 and it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein) as a glyxolytic enzyme role. The PGK1 catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate during glycolysis, generating one molecule of ATP. |
| Source : | E.coli. |
| Amino Acid Sequence : | MGSSHHHHHH SSGLVPRGSH MSLSNKLTLD KLDVKGKRVV MRVDFNVPMK NNQITNNQRI KAAVPSIKFC LDNGAKSVVL MSHLGRPDGV PMPDKYSLEP VAVELKSLLG KDVLFLKDCV GPEVEKACAN PAAGSVILLE NLRFHVEEEG KGKDASGNKV KAEPAKIEAF RASLSKLGDV YVNDAFGTAH RAHSSMVGVN LPQKAGGFLM KKELNYFAKA LESPERPFLA ILGGAKVADK IQLINNMLDK VNEMIIGGGM AFTFLKVLNN MEIGTSLFDE EGAKIVKDLM SKAEKNGVKI TLPVDFVTAD KFDENAKTGQ ATVASGIPAG WMGLDCGPES SKKYAEAVTR AKQIVWNGPV GVFEWEAFAR GTKALMDEVV KATSRGCITI IGGGDTATCC AKWNTEDKVS HVSTGGGASL ELLEGKVLPG VDALSNI. |
| Form : | Liquid. In 20 mM Tris (pH 8.0), containing 10% Glycerol, 1mM DTT. |
| Purity : | > 95% by SDS – PAGE. |
| Concentration : | 1 mg/ml (determined by Bradford assay). |
| Endotoxin Level : | < 1.0 EU per 1 μg of protein (determined by LAL method). |
| Storage : | Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles. |
| PDB rendering based on 1hdi. : |
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| Gene Name : | PGK1 phosphoglycerate kinase 1 [ Homo sapiens ] |
| Synonyms : | PGK1; phosphoglycerate kinase 1; PGKA; MIG10; MGC8947; MGC117307; MGC142128; primer recognition protein 2; cell migration-inducing gene 10 protein; EC 2.7.2.3; PRP 2; OK/SW-cl.110; OTTHUMP00000023595 |
| Gene ID : | 5230 |
| mRNA Refseq : | NM_000291 |
| Protein Refseq : | NP_000282 |
| MIM : | 311800 |
| UniProt ID : | P00558 |
| Chromosome Location : | Xq13.3 |
| Pathway : | from terpenoid and polyketide; Biosynthesis of alkaloids derived from histidine and purine; Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid; Biosynthesis of alkaloids derived from shikimate pathway; Biosynthesis of alkaloids derived; Biosynthesis of phenylpropanoids; Biosynthesis of plant hormones; Biosynthesis of terpenoids and steroids; Carbon fixation in photosynthetic organisms; Glycolysis / Gluconeogenesis; Metabolic pathways; Diabetes pathways; Integration of energy metabolism; Metabolism of carbohydrates |
| Function : | ATP binding; nucleotide binding; phosphoglycerate kinase activity; transferase activity |
◆ Recombinant Protein
| PGK1-431H | Recombinant Human PGK1 Protein, His-tagged | +Inquiry |
| PGK1-1310H | Recombinant Human PGK1 Protein, MYC/DDK-tagged | +Inquiry |
| PGK1-3391R | Recombinant Rhesus monkey PGK1 Protein, His-tagged | +Inquiry |
| PGK1-191H | Recombinant Human PGK1 protein, His-tagged | +Inquiry |
| PGK1-30893TH | Recombinant Human PGK1, His-tagged | +Inquiry |
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◆ Lysates
| PGK1-547MCL | Recombinant Mouse PGK1 cell lysate | +Inquiry |
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