Recombinant Human Hepatocyte Growth Factor

• Cat.No.: HGF-543H
• Product Overview: Recombinant Human HGF produced inBaculovirusis a heterodimer, non-glycosylated, polypeptide chain consisting an -chain of 463 amino acids and -chain of 234 having a total molecular mass of 80017 Dalton. HGF is purified by proprietary chromatographic techniques.

Specification

• Cat. No.: HGF-543H
• Description: Hepatocyte Growth Factor (HGF) is a multifunctional growth factor which regulates both cell growth and cell motility. It exerts a strong mitogenic effect on hepatocytes and primary epithelial cells. HGF synergizes with Interleukin-3 and GM-CSF to stimulate colony formation of hematopoietic progenitor cells in vitro and may, therefore, also modulate hematopoiesis.
• Source: Insect Cells.
• Amino Acid Sequence: Agrees with the sequence of native human HGF.
• Dimers And Aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
• Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
• Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Biological Activity: Recombinant Human Hepatocyte Growth Factor is fully biologically active when compared to standards. The ED50, calculated by the dose-dependant proliferation of monkey 4MBr-5 indicator cells was found to be 20-40 ng/ml.
• Endotoxin: Less than 0.1ng/µg (IEU/µg) of HGF.
• Protein Content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 1.83 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of HGF as a Reference Standard
• Formulation: The sterile protein powder is lyophilized with no additives.
• Solubility: It is recommended to reconstitute the lyophilized HGF in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Stability: Lyophilized HGF although stable at room temperature for 3 weeks, should be stored desiccated below -18℃. Upon reconstitution HGF should be stored at 4℃between 2-7 days and for future use below -18℃. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Gene Information

• Gene Name: HGF hepatocyte growth factor (hepapoietin A; scatter factor) [ Homo sapiens ]
• Synonyms: HGF; hepatocyte growth factor (hepapoietin A; scatter factor); SF; HGFB; HPTA; F-TCF; DFNB39; hepatocyte growth factor; scatter factor; hepatopoietin A; OTTHUMP00000206710; lung fibroblast-derived mitogen; fibroblast-derived tumor cytotoxic factor; deafness, autosomal recessive 39; Hepatopoeitin-A; Hepatocyte growth factor alpha chain; Hepatocyte growth factor beta chain
• Gene ID: 3082
• mRNA Refseq: NM_000601
• Protein Refseq: NP_000592
• MIM: 142409
• UniProt ID: P14210
• Chromosome Location: 7q21.1
• Pathway: Cytokine-cytokine receptor; Focal adhesion; Melanoma; Pathways in cancer; Renal cell carcinoma; Hemostasis
• Function: catalytic activity; growth factor activity; protein binding; protein heterodimerization activity; serine-type endopeptidase activity