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Recombinant S.aureus Immunoglobulin G Binding Protein A

Cat.No. : spa-515S
Product Overview : Recombinant Staphylococcus aureus Protein A was expressed inE.coliand purified by using conventional chromatography. MW =48.1 kDa (434aa).
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Cat. No. : spa-515S
Description : Protein Ais a 40-60kDasurfaceproteinoriginally found in the cell wall of thebacteriaStaphylococcus aureus. It binds with high affinity to human IgG1 and IgG2 as well as mouse IgG2a and IgG2b. Protein A binds with moderate affinity to humanIgM,IgAandIgEas well as to mouse IgG3 and IgG1. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.
Source : E.coli.
Amino Acid Sequence : MAQHDEAQQN AFYQVLNMPN LNADQRNGFI QSLKDDPSQS ANVLGEAQKL NDSQAPKADA QQNNFNKDQQ SAFYEILNMP NLNEAQRNGF IQSLKDDPSQ STNVLGEAKK LNESQAPKAD NNFNKEQQNA FYEILNMPNL NEEQRNGFIQ SLKDDPSQSA NLLSEAKKLN ESQAPKADNK FNKEQQNAFY EILHLPNLNE EQRNGFIQSL KDDPSQSANL LAEAKKLNDA QAPKADNKFN KEQQNAFYEI LHLPNLTEEQ RNGFIQSLKD DPSVSKEILA EAKKLNDAQA PKEEDNNKPG KEDNNKPGKE DNNKPGKEDG NKPGKEDNKK PGKEDNKKPG KEDNKKPGKE DGNKPGKEDN KKPGKEDGNG VHVVKPGDTV NDIAKANGTT ADKIAADNKL ADKNMIKPGQ ELVVDKKQPA NHADANKAQA LPET.
Form : Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol.
Purity : > 90% by SDS – PAGE.
Concentration : 1 mg/ml (determined by BCA assay).
Endotoxin Level : < 1.0 EU per 1 μg of protein (determined by LAL method ).
Storage : Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Gene Name : spa immunoglobulin G binding protein A [ Staphylococcus aureus subsp. aureus USA300_TCH1516 ]
Synonyms : spa; immunoglobulin G binding protein A
Gene ID : 5775424
mRNA Refseq : YP_001574038

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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Can SPA protein be used in the purification of recombinant proteins? 03/10/2023

Yes, SPA protein is effective in purifying recombinant proteins, aiding in the production of high-quality proteins for research and therapeutic applications.

Can SPA protein be employed in cancer research? 02/18/2021

Yes, SPA protein is valuable in cancer research, assisting in the identification and analysis of cancer-specific antibodies that may be potential targets for treatment.

Can SPA protein be utilized in infectious disease diagnosis? 09/15/2020

Yes, SPA protein is applied in infectious disease diagnosis to detect antibodies produced in response to pathogens, aiding in the identification of the infecting agent.

How does SPA protein enhance the accuracy of Western blotting? 06/29/2020

In Western blotting, SPA protein is used to bind and detect specific antibodies, improving the accuracy of protein identification and quantification in clinical samples.

How does SPA protein contribute to therapeutic antibody production? 10/08/2018

SPA protein is utilized in the purification process of therapeutic antibodies, helping to isolate and obtain high-purity antibodies for use in medical treatments.

Customer Reviews (3)

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Reviews
12/13/2022

    The SPA protein's superior performance in ELISA paves the way for robust and precise data analysis, providing invaluable insights into various biological processes.

    07/26/2017

      I am genuinely excited to embark on this scientific journey, empowered by the superior SPA protein and the manufacturer's unwavering commitment to ensuring my success.

      06/20/2017

        The SPA protein comes highly recommended due to its exceptional performance in ELISA assays.

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