| Cat. No. : |
KIT-149H |
| Description : |
Stem cell factor receptor (SCFR), also known as c-kit, is a member of the type III receptor tyrosine kinase (RTK) family that includes platelet-derived growth factor (PDGF) receptors and the macrophage colony-stimulating factor 1 (CSF-1) (c-fms) receptor. Soluble SCFR (SCFR) is released by human hematopoietic cells, mast cells, and endothelial cells and has been shown to bind SCF. Soluble SCF R (native or recombinant) can be a potent SCF antagonist. |
| Source : |
Human 293 cells. |
| Amino Acid Sequence : |
GSSQPSVSPGEPSPPSIHPGKSDLI VRVGDEIRLLCTDPGFVKWTFEILD ETNENKQNEWITEKAEATNTGKYTC TNKHGLSNSIYVFVRDPAKLFLVDR SLYGKEDNDTLVRCPLTDPEVTNYS LKGCQGKPLPKDLRFIPDPKAGIMI KSVKRAYHRLCLHCSVDQEGKSVLS EKFILKVRPAFKAVPVVSVSKASYL LREGEEFTVTCTIKDVSSSVYSTWK RENSQTKLQEKYNSWHHGDFNYERQ ATLTISSARVNDSGVFMCYANNTFG SANVTTTLEVVDKGFINIFPMINTT VFVNDGENVDLIVEYEAFPKPEHQQ WIYMNRTFTDKWEDYPKSENESNIR YVSELHLTRLKGTEGGTYTFLVSNS DVNAAIAFNVYVNTKPEILTYDRLV NGMLQCVAAGFPEPTIDWYFCPGTE QRCSASVLPVDVQTLNSSGPPFGKL VVQSSIDSSAFKHNGTVECKAYNDV GKTSAYFNFAFKGNNKEQIHPHT. |
| Molecular Mass : |
Under reducing conditions SCFR hcx migrates as a broad band between 65 and 100 kDa on SDS-PAGE due to post-translational modifications, in particular glycosylation. |
| pI : |
SCFR hcx separates into a number of glycoforms with a pI between 4 and 7 on 2D PAGE due to post-translational modifications, in particular glycosylation. |
| % Carbohydrate : |
Purified SCFR hcx consists of approximately 15-45% carbohydrate by weight. |
| Glycosylation : |
SCFR hcx contains N- and O-linked oligosaccharides. |
| Purity : |
>95%, as determined by SDS-PAGE, visualised by Coomassie Brilliant Blue. |
| Formulation : |
When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose. |
| Reconstitution : |
It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. |
| Storage : |
Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 4°C and longer-term storage of aliquots at -18 to -20°C is recommended. Repeated freeze thawing is not recommended. |
| Activity : |
The ED50of SCFR hcx is typically 2-4 ug/ml as by its ability to neutralise SCF mediated proliferation of the human growth dependant M-07e cell line. |
