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Recombinant Human KIT

Cat. No. : KIT-149H
Product Overview : Recombinant Human KIT encoding the signal peptide and extracellular domain of human KIT (aa 1-520) was expressed in modifiedhuman 293 cells.
Description : Stem cell factor receptor (SCFR), also known as c-kit, is a member of the type III receptor tyrosine kinase (RTK) family that includes platelet-derived growth factor (PDGF) receptors and the macrophage colony-stimulating factor 1 (CSF-1) (c-fms) receptor. Soluble SCFR (SCFR) is released by human hematopoietic cells, mast cells, and endothelial cells and has been shown to bind SCF. Soluble SCF R (native or recombinant) can be a potent SCF antagonist.
Source : Human 293 cells.
Amino Acid Sequence : GSSQPSVSPGEPSPPSIHPGKSDLIVRVGDEIRLLCTDPGFVKWTFEILDETNENKQNEWITEKAEATNTGKYTCTNKHGLSNSIYVFVRDPAKLFLVDRSLYGKEDNDTLVRCPLTDPEVTNYSLKGCQGKPLPKDLRFIPDPKAGIMIKSVKRAYHRLCLHCSVDQEGKSVLSEKFILKVRPAFKAVPVVSVSKASYLLREGEEFTVTCTIKDVSSSVYSTWKRENSQTKLQEKYNSWHHGDFNYERQATLTISSARVNDSGVFMCYANNTFGSANVTTTLEVVDKGFINIFPMINTTVFVNDGENVDLIVEYEAFPKPEHQQWIYMNRTFTDKWEDYPKSENESNIRYVSELHLTRLKGTEGGTYTFLVSNSDVNAAIAFNVYVNTKPEILTYDRLVNGMLQCVAAGFPEPTIDWYFCPGTEQRCSASVLPVDVQTLNSSGPPFGKLVVQSSIDSSAFKHNGTVECKAYNDVGKTSAYFNFAFKGNNKEQIHPHT.
Molecular Mass : Under reducing conditions SCFR hcx migrates as a broad band between 65 and 100 kDa on SDS-PAGE due to post-translational modifications, in particular glycosylation.
pI : SCFR hcx separates into a number of glycoforms with a pI between 4 and 7 on 2D PAGE due to post-translational modifications, in particular glycosylation.
% Carbohydrate : Purified SCFR hcx consists of approximately 15-45% carbohydrate by weight.
Glycosylation : SCFR hcx contains N- and O-linked oligosaccharides.
Purity : >95%, as determined by SDS-PAGE, visualised by Coomassie Brilliant Blue.
Formulation : When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
Reconstitution : It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
Storage : Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 4°C and longer-term storage of aliquots at -18 to -20°C is recommended. Repeated freeze thawing is not recommended.
Activity : The ED50of SCFR hcx is typically 2-4 ug/ml as by its ability to neutralise SCF mediated proliferation of the human growth dependant M-07e cell line.
Gene Name : KIT v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog [ Homo sapiens ]
Synonyms : KIT; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; PBT; SCFR; C-Kit; CD117; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; soluble KIT variant 1; mast/stem cell growth factor receptor; proto-oncogene tyrosine-protein kinase Kit; EC 2.7.10.1
Gene ID : 3815
mRNA Refseq : NM_000222
Protein Refseq : NP_000213
UniProt ID : P10721
Chromosome Location : 4q11-q12
MIM : 164920
Pathway : Acute myeloid leukemia; Cytokine-cytokine receptor interaction; Endocytosis; Hematopoietic cell lineage; Melanogenesis; Pathways in cancer
Function : ATP binding; nucleotide binding; protein binding; receptor signaling protein tyrosine kinase activity; stem cell factor receptor activity; transferase activity

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