Active Recombinant Human PVRL2 protein, His-tagged

• Cat.No.: PVRL2-609H
• Product Overview: Recombinant Human PVRL2 protein(Gln 32 - Leu 360), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
• Bio-activity: Immobilized Human PVRIG, Mouse IgG2a Fc Tag, low endotoxin at 2 μg/mL (100 μL/well) can bind Human Nectin-2, His Tag with a linear range of 0.078-0.625 μg/mL. Immobilized Human TIGIT, Fc Tag at 10 μg/mL (100 μL/well) can bind Human Nectin-2, His Tag with a linear range of 0.625-5 μg/mL. Immobilized Human PVRIG, Fc Tag (at 5 μg/mL (100 μL/well) can bind Human Nectin-2, His Tag with a linear range of 0.039-0.625 μg/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 37.4 kDa. The protein migrates as 45-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein Length: Gln 32 - Leu 360
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: PVRL2 poliovirus receptor-related 2 (herpesvirus entry mediator B) [ Homo sapiens ]
• Official Symbol: PVRL2
• Synonyms: PVRL2; poliovirus receptor-related 2 (herpesvirus entry mediator B); HVEB; poliovirus receptor-related protein 2; CD112; PRR2; PVRR2; nectin-2; poliovirus receptor-like 2; herpesvirus entry protein B
• Gene ID: 5819
• mRNA Refseq: NM_001042724
• Protein Refseq: NP_001036189
• MIM: 600798
• UniProt ID: Q92692