Creative BioMart to Present at
                        BIO-Europe Spring Creative BioMart to Present at AACR Annual Meeting|Apr. 5-10, 2024|Booth #2953

Active Recombinant Human FXI protein, His-tagged

Cat.No. : F11-290H
Product Overview : Recombinant Human FXI protein(Glu 19 - Val 625), fused with His tag, was expressed in HEK293.
Unit
Price
Qty
  • Specification
  • Gene Information
  • Related Products
Source : HEK293
Species : Human
Tag : His
Form : Lyophilized from 0.22 μm filtered solution in 25 mM HEPES, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Bio-activity : Measured by its ability to cleave the fluorogenic peptide substrate, t-butyloxycarbonyl-Ile-Glu-Gly-Arg-7-amido-4-methylcoumarin (Boc-IEGR-AMC). The specific activity is >200 pmol/min/µg
Molecular Mass : This protein carries a polyhistidine tag at the C-terminus. The proprotein is activated by factor XIIa (or XII) and cleaved after Arg-387 into the light chain with calculated MW of 28.7 kDa, which contains the active site, and the heavy chain with calculated MW of 41.2 kDa. The protein migrates as 47 kDa (heavy chain) and 35 kDa (light chain) under reducing (R) condition (SDS-PAGE) due to glycosylation.
Proteinlength : Glu19-Val625
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >90% as determined by SDS-PAGE.
Storage : For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (0)

Ask a question

Customer Reviews (0)

Write a review

Ask a Question for All FXI Products

Required fields are marked with *

My Review for All FXI Products

Required fields are marked with *

0

Inquiry Basket

cartIcon
logo

FOLLOW US

Terms and Conditions        Privacy Policy

Copyright © 2024 Creative BioMart. All Rights Reserved.

Contact Us

  • /

Stay Updated on the Latest Bioscience Trends