Active Recombinant Human CEACAM1 protein(Met1-Gly428), His&hFc-tagged

• Cat.No.: CEACAM1-663H
• Product Overview: Recombinant Human CEACAM1 (NP_001020083.1) (Met 1-Gly 428) was expressed in HEK293, fused with the C-terminal polyhistidine-tagged Fc region of Human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: C-His&hFc
• Protein length: Met1-Gly428
• Form: Lyophilized from sterile 100 mM Glycine, 500 mM NaCl, 5 % Glycerol, 50 mM Arg Hcl, 50 mM Glu, 0.01 % tween 20, pH 5.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized CEACAM8-His at 10 μg/mL (100 μL/well) can bind CEACAM1-Fch, the EC50 of CEACAM1-Fch is 70-200 ng/mL.
• Molecular Mass: The secreted recombinant human CEACAM1/Fc chimera is a disulfide-linked homodimer. The reduced monomer comprises 642 amino acids and predicts a molecular mass of 71.4 kDa. As a result of glycosylation, the apparent molecular mass of rhCEACAM1 is approximately 124.8 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CEACAM1 carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein) [ Homo sapiens ]
• Official Symbol: CEACAM1
• Synonyms: CEACAM1; carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein); BGP; carcinoembryonic antigen-related cell adhesion molecule 1; BGP1; CD66a; antigen CD66; CD66a antigen; BGPI
• Gene ID: 634
• mRNA Refseq: NM_001024912
• Protein Refseq: NP_001020083
• MIM: 109770
• UniProt ID: P13688