Active Recombinant Human DIABLO protein, His-tagged

• Cat.No.: DIABLO-869H
• Product Overview: Recombinant Human DIABLO protein(NP_063940.1)(Ala56-Asp239), fused with His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized recombinant human SMAC-His at 10 μg/ml (100 μl/well) can bind recombinant human XIAP-AVI with a linear range of 0.125-1.0 μg/ml.
• Molecular Mass: The recombinant human Diablo/SMAC consists of 191 amino acids and migrates as polypeptide of 22 kDa as estimated by SDS-PAGE under reduced and non-reduced conditions.
• Protein Length: Ala56-Asp239
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: DIABLO diablo, IAP-binding mitochondrial protein [ Homo sapiens ]
• Official Symbol: DIABLO
• Synonyms: DIABLO; diablo, IAP-binding mitochondrial protein; diablo homolog, mitochondrial; DFNA64; DIABLO S; FLJ10537; FLJ25049; second mitochondria derived activator of caspase; SMAC; 0610041G12Rik; mitochondrial Smac protein; direct IAP-binding protein with low pI; second mitochondria-derived activator of caspase; SMAC3; DIABLO-S
• Gene ID: 56616
• mRNA Refseq: NM_019887
• Protein Refseq: NP_063940
• MIM: 605219
• UniProt ID: Q9NR28