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Active Recombinant Mouse Thpo protein, His-tagged

Cat.No. : Thpo-97M
Product Overview : Recombinant Mouse Thpo protein(NP_033405.1)(Met1-Thr356), fused with His tag, was expressed in HEK293.
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Source : HEK293
Species : Mouse
Tag : His
Form : Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Bio-activity : Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is typically 1.6-6.4 ng/mL.
Molecular Mass : The recombinant mouse THPO consists of 346 amino acids with the predicted molecular mass of 37 kDa. As a result of glycosylation, rmTHPO migrates as an approximately 80-90 kDa band in SDS-PAGE under reducing conditions.
Proteinlength : Met1-Thr356
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method
Purity : > 90 % as determined by SDS-PAGE
Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Gene Name : Thpo thrombopoietin [ Mus musculus ]
Official Symbol : Thpo
Synonyms : THPO; thrombopoietin; C-mpl ligand; megakaryocyte colony-stimulating factor; megakaryocyte growth and development factor; myeloproliferative leukemia virus oncogene ligand; Ml; Tpo; Mgdf; Tpo1; Tpo2; Tpo3; Tpo4; Mpllg;
Gene ID : 21832
mRNA Refseq : NM_001173505
Protein Refseq : NP_001166976

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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