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Recombinant Mouse Chemokine (C-C Motif) Ligand 22

Cat. No.: Ccl22-91M
Product Overview: Recombinant Mouse chemokine (C-C motif) ligand 22 is produced inE. Coli.
Description: Murine Macrophage-Derived Chemokine (MDC) is a unique member of the CC chemokine family. Human MDC is highly expressed in macrophages and in monocyte-derived dendritic cells, but not in monocytes, natural killer cells, or several cell lines of epithelial, endothelial or fibroblast origin. This recombinant version is a peptide of 7.8 kDa that consists of 68 amino acid residues.
Source: E. Coli.
Amino-Acid Sequence: GPYGANVEDS ICCQDYIRHP LPSRLVKEFF WTSKSCRKPG VVLITVKNRD ICADPRQVWV KKLLHKLS.
Supplied: Powder with no additives.
Purity: > 98% by SDS-Page and HPLC analysis Endotoxin level is less than 0.1ng per ug (1EU/mg) of MDC.
Reconstitution: The MDC should be reconstituted in water to a concentration of 0.1-1.0mg/ml. Allow to set at least 30 minutes at 4 ℃, tighten cap and rotate to reconstitute any material on sides of vial. If desired, you can centrifuge at 1000 rpm for 1 minute to concentrate volume in bottom of vial. This solution can be diluted into other buffered solutions containing carrier protein or medium and stored at 4 ℃ for up to one week or at -20 ℃ for future use.
Note: If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >50ug/ml.
Reactivity: The reactivity of this murine MDC was determined by a chemotaxis assay using human activated lymphocytes. Significant chemotaxis was achieved using a concentration of 10-100ng/ml.
Storage: The powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 ℃. Reconstituted MDC should be stored in working aliquots at -20℃. Avoid Frequent Freeze-Thaw Cycles.
Gene Name: Ccl22 chemokine (C-C motif) ligand 22 [ Mus musculus ]
Synonyms: Ccl22; chemokine (C-C motif) ligand 22; ABCD-1; DCBCK; MDC; Scya22; OTTMUSP00000017971; SMALL INDUCIBLE CYTOKINE A22 PRECURSOR (CC CHEMOKINE ABCD-1) (ACTIVATED B AND DENDRITIC CELL-DERIVED); dendritic cell and B cell derived chemokine; small inducible cytokine subfamily A, member 22; small inducible cytokine subfamily A22
Gene ID: 20299
mRNA Refseq: NM_009137
Protein Refseq: NP_033163
UniProt ID: O88430
Chromosome Location: 8 C5
Pathway: Chemokine signaling pathway; Cytokine-cytokine receptor interaction
Function: chemokine activity; cytokine activity

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