||Like most bacteria, Escherichia coli expresses a single MSRB (Methionine-R-sulfoxide reductase, EC18.104.22.168; accession number NP416292) that carries out the reduction of methionine-R-sulfoxide to methionine. The E. coli enzyme – like the two human enzymes offered by Jena Bioscience - belongs to a group of MsrBs, that possess a metal binding site composed of two CXXC motifs. The bound metal (zinc or iron) may stabilize the conformation of the enzymes. MW = 16.8 kDa.
||Liquid. Supplied in TRIS-HCl pH 7.4 and 33% glycerol.
||>90% by SDS-PAGE.
||1 nmol of E. coli MSRB will reduce 0.6 nmol peptidebound Met-S-sulfoxide in 1 min at 37°C.
||MetO-containing peptides are reduced by the enzyme in the presence of DTT.
||For in vitro use only.
||Quality guaranteed for 12 months store at - 80°C. Avoid freeze / thaw cycles.