||Liver X Receptors (LXRs) are nuclear receptors that regulate the metabolism of cholesterol and bile acids. There are two subtypes of LXRs, LXRα and LXRβ. LXRα is preferentially expressed in liver, small intestine, kidney and spleen. In contrast, LXRβ expression is ubiquitous. The genomic structure and the promoter regions of the two LXR genes contain specific regulatory sites, which suggest that LXRs may have physiological roles in the immune system. Like other nuclear receptors, LXRs heterodimerize with Retinoid X Receptor (RXR) for function. LXRs are activated by naturally occurring oxysterols and regulate the expression of target genes, including ATP binding cassette transporter 1 (ABC1), ATP binding cassette transporter 8 (ABC8) and cholesterol ester transfer protein (CETP). LXR is thought to play a major role in the control of cholesterol catabolism by stimulating the expression of cholesterol 7alpha-hydroxylase (CYP7A1), the rate limiting enzyme of bile acid synthesis. Recombinant LXR is isolated from anE. colistrain that carries the coding sequence of the human LXRα under the control of a T7 promoter.
||LXR has been applied in DNA and protein-protein interactions assays.
||For in vitro use only.
||Liquid. Supplied in 20 mM Tris-HCl pH 8.0, 100 mM KCl, 0.2 mM EDTA, 1 mM DTT and 20% glycerol.
||20 ng are sufficient for a gel-mobility shift assay and 100 ng are sufficient for a protein-protein interaction assay.
||> 95% by SDS-PAGE.
||Quality guaranteed for 12 months, Store at -80°C. Avoid freeze / thaw cycles.