"SFRS1" Related Products

Recombinant Human Splicing Factor, Arginine/Serine-rich 1

Cat. No.: SFRS1-1014H
Description: ASF or called SF2, a member of SR protein family, is an essential pre-mRNA splicing factor required for both single and alternative splicing. Phosphorylation on serine residues located within the SR domain directly regulates ASF/SF2 activity and compartmentalization of other SR splicing factors. In addition to interacting with RNA and other splicing factors, such as U1-70K, U2AF and other SR proteins, ASF/SF2 also directly or indirectly interacts with HIV regulatory protein Rev, the C-terminal domain (CTD) of the largest subunit of RNA polymerase II, and numerous transcription factors, thereby suggesting a potential role of ASF/SF2 in coordinating of transcription and pre-mRNA splicing. The human ASF/SF2 wild type protein (residues 1-248) was expressed in E. coli and purified by an affinity column.
Source: E. coli.
Form: Liquid. Supplied in 20 mM Tris-HCl, pH 8.0, 100 mM KCl, 0.2 mM EDTA, 1 mM DTT, 20% glycerol.
Purity: >95% by SDS-PAGE.
Activity: 1 ng is sufficient for a gel mobility shift assay in a 20 μl reaction and 100 ng are sufficient for a protein-protein interaction assay.
Application: Recombinant ASF/SF2 protein can be used 1) for in vitro function studies including pre-mRNA splicing, cross linking and other RNA binding assays.2) for protein-protein interaction assay, and 3) for cell growth and proliferation assays.
Usage: For in vitro use only.
Storage: Quality guaranteed for 12 months store at -80°C. Avoid freeze / thaw cycles.
Gene Name: SFRS1 splicing factor, arginine/serine-rich 1 [ Homo sapiens ]
Synonyms: SFRS1; splicing factor, arginine/serine-rich 1; ASF; SF2; SF2p33; SRp30a; MGC5228; splicing factor 2; pre-mRNA-splicing factor SF2, P33 subunit; alternate splicing factor; ASF-1; SF2P33; SRp30a
Gene ID: 6426
mRNA Refseq: NM_001078166
Protein Refseq: NP_001071634
MIM: 600812
UniProt ID: Q07955
Chromosome Location: 17q21.3-q22
Function: RNA binding; nucleotide binding; protein binding

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