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Recombinant Human OGN, His-tagged

Cat.No. : OGN-914H
Product Overview : A DNA sequence encoding the mature form of human Osteoglycin (Ala20 - Phe298) with 6 His tag on the N-Terminus was expressed in E. Coli. This protein was purified by Ni-NTA column.
Description : This gene encodes a protein which induces ectopic bone formation in conjunction with transforming growth factor beta. This protein is a small proteoglycan which contains tandem leucine-rich repeats (LRR). Alternatively spliced transcript variants that encode the same protein have been found for this gene.
Source : E. Coli
Species : Human
Tag : His
Form : Lyophilized 100 µg of human Osteoglycin in 100 ul of PBS. Carry free.
Molecular Mass : 19 KD
AA Sequence : APPTQQDSRII YDYGTDNFEE SIFSQDYEDK YLDGKNIKEK ETVIIPNEKS LQLQKDEAIT PLPPKKENDE MPTCLLCVCL SGSVYCEEVD IDAVPPLPKE SAYLYARFNK IKKLTAKDFA DIPNLRRLDF TGNLIEDIED GTFSKLSLLE ELSLAENQLL KLPVLPPKLT LFNAKYNKIK SRGIKANAFK KLNNLTFLYL DHNALESVPL NLPESLRVIH LQFNNIASIT DDTFCKANDT SYIRDRIEEI RLEGNPIVLG KHPNSFICLK RLPIGSYF
Purity : >95% in SDS-PAGE gel
Applications : ELISA
Storage : Store lyophilized protein at -20 °C or -70 °C. Lyophilized protein is stable for up to 6 months from date of receipt at -20 °C to -70 °C. Upon reconstitution, this protein can be stored at -20 °C for a few weeks or at -70 °C in a manual defrost freezer for long term storage (six months). Aliquot reconstituted protein to avoid repeated freezing / thawing cycles.
Reconstitution : Add 500 ul deionized water to the vial to prepare a working stock solution at 200 ug/mL. Allow to set at least 30 minutes at 4 °C, mix well.
Gene Name : OGN osteoglycin [ Homo sapiens (human) ]
Official Symbol : OGN
Synonyms : OGN; OG; OIF; SLRR3A; osteoglycin; mimecan; mimecan proteoglycan; osteoinductive factor; corneal keratan sulfate proteoglycan
Gene ID : 4969
mRNA Refseq : NM_014057
Protein Refseq : NP_054776
MIM : 602383
UniProt ID : P20774
Chromosome Location : 9q22
Pathway : Glycosaminoglycan metabolism; Keratan sulfate biosynthesis; MPS I - Hurler syndrome
Function : growth factor activity; protein binding

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