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Active Recombinant Human General Transcription Factor IIA, 1, 19/37kDa, P55

Cat.No. : GTF2A1-1175H
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Cat. No. : GTF2A1-1175H
Description : The Transcription Factor IIA (TFIIA) has been shown to bind to the TBP-DNA complex and to increase the affinity of TBP for the TATA element. Human TFIIA consists of three subunits of 35 kDa (α-subunit), 19 kDa (β-subunit) and 12 kDa (γ-subunit). The α- and β-subunits are derived from the product, p55, of a single gene by an unknown mechanism. However, recombinant p55, in combination with a 12 kDa subunit (γ-subunit), retains native TFIIA activity. P55 subunit of TFIIA is isolated form a strain ofE. colithat contains the coding sequence of human p55 of TFIIA under the control of T7 promoter. Recombinant TFIIA can be used to super-shift TBPDNA complex in a gel mobility shift assay and to stimulate activator-dependent transcriptionin vitro.
Source : E. coli.
Usage : For in vitro use only.
Form : Liquid. Supplied in 20 mM Tris-HCl pH 8.0, 100 mM KCl, 0.2 mM EDTA, 1 mM DTT and 20% glycerol.
Activity : 1 ng is sufficient for a gel mobility shift assay in a 20 μ l reaction, 20 ng are sufficient for in vitro transcription assay and 100 ng are sufficient for protein-protein interaction assays.
Purity : > 95% by SDS-PAGE.
Storage : Quality guaranteed for 12 months, store at -80℃. Avoid freeze / thaw cycles.
Gene Name : GTF2A1 general transcription factor IIA, 1, 19/37kDa [ Homo sapiens ]
Synonyms : GTF2A1; general transcription factor IIA, 1, 19/37kDa; TF2A1; TFIIA; MGC129969; MGC129970; TFIIA alpha, p55, isoform 1; glucose regulated protein, 58kD pseudogene; TFIIA-42;Transcription initiation factor IIA subunit 1; Transcription initiation factor IIA alpha chain; TFIIA p35 subunit; TFIIA p19 subunit
Gene ID : 2957
mRNA Refseq : NM_015859
Protein Refseq : NP_056943
MIM : 600520
UniProt ID : P52655
Chromosome Location : 14q31
Pathway : Basal transcription factors; Gene Expression; HIV Infection; Transcription
Function : DNA binding; TATA-binding protein binding; general RNA polymerase II transcription factor activity; protein heterodimerization activity; transcription coactivator activity; transcription factor binding

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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