||The TATA-binding protein (TBP) is believed to function as an essential factor of the general transcription machinery and to be involved in transcription by all three eukaryotic RNA Polymerases (Pol I, II, and III). TBP specifically binds to the TATA element at the promoter region and interacts with numerous transcription factors, including TBP-associated factors (TAFs), activators, and some tumor suppressor proteins. Isolated from anE. colistrain that carries the coding sequence for human TBP under the control of T7 promoter. TBP can be used for: (1) in vitro transcription; (2) gel mobility shift assay in the presence of doublestranded oligonucleotide containing a TATA element; (3) for in vitro footprinting assay in the presence of TATAcontaining DNA fragment and (4) for protein-protein interaction assay. Protein is greater than 95% homogeneous and contains no detectable proteases, DNase, and RNase activity.
||For in vitro use only.
||Liquid. Supplied in 20 mM Tris-HCl pH 8.0, 100 Mm KCl, 0.2 mM EDTA, 1 mM DTT and 20% glycerol.
||1 ng is the amount sufficient for a gel mobility shift assay in a 20 μl reaction. 10 ng are sufficient for a 25μl reconstituted transcription reaction and 100 ng are sufficient for a protein-protein interaction assay detected by immuno-blot system.
||> 95% by SDS-PAGE.
||Quality guaranteed for 12 months, store at -80°C. Avoid freeze / thaw cycles.