"TOP1" Related Products


Recombinant Human Topoisomerase (DNA) I, N-terminal Domain

Cat. No. : TOP1-1197H
Description : Human DNA Topoisomerase I is the best studied of the DNA topoisomerase family. It catalyzes the relaxation of both positive and negative supercoils without the requirement of energy. In addition to DNA replication and transcriptional activation, DNA Topoisomerase I also plays a major role in pre-mRNA splicing, cell cycle, and other gene regulatory pathways during cell growth and development. The N-terminal 214 amino acids of Topoisomerase I comprise a highly charged N-terminal domain (NTD) involved in protein-protein interactions with a number of cellular proteins, including SV40 T antigen, nucleolin, SR proteins, p53 and other transcription factors. The N-terminal domain (NTD) of human DNA Topoisomerase I protein (residue 1-197) was expressed in baculovirus system and purified by using an affinity column and FPLC chromatography. Purified Topo I NTD can be tested forin vitrotranscription, pre-mRNA splicing, DNA binding and protein-protein interaction assays. Purified Topo I protein (NTD) is greater than 95% homogeneous and contains no detectable protease, DNase, and RNase activity.
Source : Sf9 insect cells.
Form : Liquid. Supplied in 20 mM Tris-HCl, pH 8.0, 100 mM KCl, 0.2 mM EDTA, 1 mM DTT, 20 % glycerol.
Purity : > 95% by SDS-PAGE.
Activity : 0.1-10 ng have been tested for in vitro relaxation assay in a 20 μl reaction and contain no detectable DNA relaxation activity.
Usage : For in vitro use only.
Storage : Quality guaranteed for 12 months store at -80°C. Avoid freeze / thaw cycles.
Gene Name : TOP1 topoisomerase (DNA) I [ Homo sapiens ]
Synonyms : TOP1; topoisomerase (DNA) I; TOPI; DNA topoisomerase1; EC; OTTHUMP00000031713; DNA topoisomerase I; topoisomerase I; type I DNA topoisomerase
Gene ID : 7150
mRNA Refseq : NM_003286
Protein Refseq : NP_003277
MIM : 126420
UniProt ID : P11387
Chromosome Location : 20q12-q13.1
Function : ATP binding; DNA topoisomerase (ATP-hydrolyzing) activity; DNA topoisomerase type I activity; chromatin binding; nucleotide binding; protein binding

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