Active Recombinant Human vitamin D receptor

• Cat.No.: VDR-1204H

Specification

• Cat. No.: VDR-1204H
• Description: The vitamin D endocrine system is critical for the proper development and maintenance of mineral ion homeostasis and skeletal integrity. Beyond these classical roles, recent evidence suggests that the bioactive metabolite of vitamin D, 1, 2, 5- dihydroxyvitamin D3, functions in diverse physiological processes, such as hair follicle cycling, blood pressure regulation, and mammary gland development. The biological effects of 1, 2, 5-(OH)2D3 are mediated through the vitamin D receptor (VDR), a member of the nuclear receptor superfamily of ligand-activated transcription factors. The cellular effects of VDR signaling include growth arrest, differentiation and/or induction of apoptosis. VDR heterodimerizes with RXR and the liganded VDR-RXR heterodimer binds with high affinity to vitamin D response elements (VDREs) in the promoters of target genes. In addition, several nuclear receptor coactivators (SRC-1, DRIP) have been shown to interact with VDR and potentiate its transcriptional activity. In addition to treating disorders of mineral metabolism and diseases of the skeleton, such as rickets, osteoporosis, and renal osteodystrophy, VDR and 1, 2, 5-(OH)2D3 have significant therapeutic potential for pathologies such as cancer, autoimmune syndromes, and psoriasis.
• Source: E. coli.
• Form: Liquid. Supplied in 20 mM Tris-HCl pH 8.0, 100 mM KCl, 0.2 mM EDTA, 1 mM DTT and 20% glycerol.
• Purity: >95% by SDS-PAGE.
• Activity: 1-5 ng are sufficient for a gel mobility shift assay in a 20 μl reaction, 50-100 ng are sufficient for reconstituted transcription assay and 100-200 ng are sufficient for a protein-protein interaction assay or an acetylation assay.
• Usage: For in vitro use only.
• Storage: Quality guaranteed for 12 months store at -80°C. Avoid freeze / thaw cycles.

Gene Information

• Gene Name: VDR vitamin D (1,25-dihydroxyvitamin D3) receptor [ Homo sapiens ]
• Synonyms: VDR; vitamin D (1,25-dihydroxyvitamin D3) receptor; NR1I1; 1,25-dihydroxyvitamin D3 receptor; Nuclear receptor subfamily 1 group I member 1
• Gene ID: 7421
• mRNA Refseq: NM_000376
• Protein Refseq: NP_000367
• MIM: 601769
• UniProt ID: P11473
• Chromosome Location: 12q12-q14
• Pathway: Gene Expression
• Function: metal ion binding; protein binding; retinoid X receptor binding; sequence-specific DNA binding; steroid hormone receptor activity; transcription factor activity; vitamin D response element binding; vitamin D3 receptor activity; zinc ion binding