||Recombinant human PEA-15 was expressed inE.coliand purified by using conventional chromatography. MW = 15 kDa (130aa).
||Phospho-enriched protein in astrocytes 15 kDa (PEA-15) is a death effector domain (DED)-containing protein predominantly expressed in the central nervous system, particularly in astrocytes. This protein has been implicated in the regulation of multiple cellular processes including apoptosis, proliferation, glucose transport, adhesion and migration. Increased PEA-15 levels may affect tumorigenesis and cancer progression, thus it is overexpressed in breast cancers and gliomas as well as in type 2 diabetes.
|Amino Acid Sequence:
||MAEYGTLLQD LTNNITLEDL EQLKSACKED IPSEKSEEIT TGSAWFSFLE SHNKLDKDNL SYIEHIFEIS RRPDLLTMVV DYRTRVLKIS EEDELDTKLT RIPSAKKYKD IIRQPSEEEI IKLAPPPKKA
||Liquid. In 20 mM Tris-HCl buffer (pH 7.5) containing 1 mM DTT, 10% Glycerol.
||> 90% by SDS – PAGE.
||1 mg/ml (determined by Bradford assay).
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.