Recombinant Human Vacuolar Protein Sorting 28 Homolog (S. cerevisiae)

• Cat.No.: VPS28-1407H
• Product Overview: Recombinant human VPS28 was expressed inE.coliand purified by using conventional chromatography. MW = 25.4 kDa (221aa).

Specification

• Cat. No.: VPS28-1407H
• Description: VPS28 is involved in endosomal sorting of cell surface receptors via a multivesicular body/late endosome pathway. This protein is a subunit of the ESCRT-I complex (endosomal complexes required for transport) involved in the sorting of ubiquitinated proteins. VSP28binding with the TSG101 may play important roles in budding of Retroviruses, such as HIV-1 and Ebola virus.
• Source: E.coli.
• Amino Acid Sequence: MFHGIPATPG IGAPGNKPEL YEEVKLYKNA REREKYDNMA ELFAVVKTMQ ALEKAYIKDC VSPSEYTAAC SRLLVQYKAA FRQVQGSEIS SIDEFCRKFR LDCPLAMERI KEDRPITIKD DKGNLNRCIA DVVSLFITVM DKLRLEIRAM DEIQPDLREL METMHRMSHL PPDFEGRQTV SQWLQTLSGM SASDELDDSQ VRQMLFDLES AYNAFNRFLH A
• Form: Liquid. In 20 mM Tris-HCl buffer (pH 7.5) containing 1 mM DTT, 10% Glycerol
• Purity: > 95% by SDS – PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Endotoxin Level: < 1.0 EU per 1 μg of protein (determined by LAL method).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: VPS28 vacuolar protein sorting 28 homolog (S. cerevisiae) [ Homo sapiens ]
• Synonyms: VPS28; vacuolar protein sorting 28 homolog (S. cerevisiae); MGC60323;yeast class E protein Vps28p homolog; vacuolar protein sorting 28 (yeast); H-Vps28; ESCRT-I complex subunit VPS28; vacuolar protein sorting 28; Vacuolar protein sorting-associated protein 28 homolog
• Gene ID: 51160
• mRNA Refseq: NM_016208
• Protein Refseq: NP_057292
• MIM: 611952
• UniProt ID: Q9UK41
• Chromosome Location: 8q24.3
• Pathway: Endocytosis
• Function: protein binding