Cat.No. :	ROCK1-420H
Product Overview :	Recombinant human ROCK1 (17-535) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Description :	This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity.
Source :	Sf9 insect cells using baculovirus
Species :	Human
Tag :	GST
Form :	Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
Bio-activity :	65 nmol/min/mg
Molecular Mass :	~85 kDa
Protein length :	17-535
Purity :	>90%
Applications :	Kinase Assay, Western Blot
Stability :	1 yr at -70°C from date of shipment.
Storage :	Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Concentration :	0.1 ug/ul

Gene Name :	ROCK1 Rho-associated, coiled-coil containing protein kinase 1 [ Homo sapiens (human) ]
Official Symbol :	ROCK1
Synonyms :	ROCK1; Rho-associated, coiled-coil containing protein kinase 1; PRO0435; MGC43611; P160ROCK; MGC131603; p160-ROCK; Rho kinase; renal carcinoma antigen NY-REN-35; EC 2.7.11.1
Gene ID :	6093
mRNA Refseq :	NM_005406
Protein Refseq :	NP_005397
MIM :	601702
UniProt ID :	Q13464
Chromosome Location :	18q11.1
Function :	ATP binding; GTP-Rho binding; metal ion binding; protein binding

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