Recombinant Human S100 Calcium Binding Protein B, His-tagged

• Cat.No.: S100B-2724H
• Product Overview: Recombinant human S100B protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. MW = 12.8 kDa (112aa), confirmed by MALDI-TOF.

Specification

• Cat. No.: S100B-2724H
• Description: S100B is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. This protein function in Neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly.
• Source: E.coli.
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MSELEKAMVA LIDVFHQYSG REGDKHKLKK SELKELINNE LSHFLEEIKE QEVVDKVMET LDNDGDGECD FQEFMAFVAM VTTACHEFFE HE
• Form: Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 10% glycerol.
• Purity: > 90% by SDS – PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: S100B S100 calcium binding protein B [ Homo sapiens ]
• Synonyms: S100B; S100 calcium binding protein B; NEF; S100; S100 calcium-binding protein, beta; S100 beta; OTTHUMP00000174958; S-100 calcium-binding protein, beta chain; S100 calcium binding protein, beta (neural); S100 calcium-binding protein, beta (neural); Protein S100-B; S100 calcium-binding protein B; S-100 protein subunit beta; S-100 protein beta chain
• Gene ID: 6285
• mRNA Refseq: NM_006272
• Protein Refseq: NP_006263
• MIM: 176990
• UniProt ID: P04271
• Chromosome Location: 21q22.3
• Function: S100 beta binding; calcium ion binding; calcium-dependent protein binding; protein homodimerization activity; protein homodimerization activity; tau protein binding; zinc ion binding