||Recombinant full length Human DUSP3; amino acids 1-185, 20.5kDa.
||The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene maps in a region that contains the BRCA1 locus which confers susceptibility to breast and ovarian cancer. Although DUSP3 is expressed in both breast and ovarian tissues, mutation screening in breast cancer pedigrees and in sporadic tumors was negative, leading to the conclusion that this gene is not BRCA1.
||Specific activity:1 nmol of Cdc25A will hydrolyze 180 nmol of pNPP per minute at 37°C at pH 6.5.Assay buffer:25 mM MOPS, pH 6.5 and 5 mM EDTA.
||>90% by SDS-PAGE
||Preservative: NoneConstituents: 50mM HEPES, 40mM Sodium chloride, 1mM EDTA, 1mM DTT, pH 7.4
||Shipped on dry ice. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
||Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.Contains 1 tyrosine-protein phosphatase domain.