Recombinant Human EEF1D, His-tagged

• Cat.No.: EEF1D-28466TH
• Product Overview: Recombinant full length Human EEF1D with an N terminal His tag; 301 amino acids with a predicted MWt 33.2 kDa including tag.

Specification

• Description: This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit, delta, functions as guanine nucleotide exchange factor. It is reported that following HIV-1 infection, this subunit interacts with HIV-1 Tat. This interaction results in repression of translation of host cell proteins and enhanced translation of viral proteins. Several alternatively spliced transcript variants encoding multiple isoforms have been found for this gene. Related pseudogenes have been defined on chromosomes 1, 6, 7, 9, 11, 13, 17, 19.
• Protein length: 281 amino acids
• Conjugation: HIS
• Molecular Weight: 33.200kDa inclusive of tags
• Source: E. coli
• Form: Liquid
• Purity: >90% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 10% Glycerol, 20mM Tris HCl, 1mM DTT, pH 8.0
• Storage: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Sequences of amino acids: MGSSHHHHHHSSGLVPRGSHMATNF LAHEKIWFDKFKYDDAERRFYEQMN GPVAGASRQENGASVILRDIARARE NIQKSLAGSSGPGASSGTSGDHGEL VVRIASLEVENQSLRGVVQELQQAI SKLEARLNVLEKSSPGHRATAPQTQ HVSPMRQVEPPAKKPATPAEDDEDD DIDLFGSDNEEEDKEAAQLREERLR QYAEKKAKKPALVAKSSILLDVKPW DDETDMAQLEACVRSIQLDGLVWGA SKLVPVGYGIRKLQIQCVVEDDKVG TDLLEEEITKFEEHVQSVDIAAFNK I
• Sequence Similarities: Belongs to the EF-1-beta/EF-1-delta family.

Gene Information

• Gene Name: EEF1D eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein) [ Homo sapiens ]
• Official Symbol: EEF1D
• Synonyms: EEF1D; eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein); elongation factor 1-delta; EF 1D; FLJ20897
• Gene ID: 1936
• mRNA Refseq: NM_001130053
• Protein Refseq: NP_001123525
• MIM: 130592
• Uniprot ID: P29692
• Chromosome Location: 8q24
• Pathway: Eukaryotic Translation Elongation, organism-specific biosystem; Gene Expression, organism-specific biosystem; Herpes simplex infection, organism-specific biosystem; Herpes simplex infection, conserved biosystem; Metabolism of proteins, organism-specific biosystem
• Function: protein binding; signal transducer activity; translation elongation factor activity; translation factor activity, nucleic acid binding