Active Recombinant Human ENO2

• Cat.No.: ENO2-28411TH
• Product Overview: Recombinant NSE was expressed in E.coli and purified by conventional chromatography techniques.

Specification

• Description: This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.
• Source: E. coli
• Species: Human
• Form: Liquid in 20 mM Tris pH 7.5, 0.1 M KCl, 5 mM MgSO4
• Bio-activity: Specific activity: > 8 units/ml. One unit will convert 1.0 umole of 2-phosphoglycerate to phospho(enol)pyruvate per minute at pH7.5 at 25°C.
• Molecular Mass: 47 kDa ( 434 aa), confirmed by MALDI-TOF.
• Protein length: 1-434aa
• AA Sequence: MSIEKIWARE ILDSRGNPTV EVDLYTAKGL FRAAVPSGAS TGIYEALELR DGDKQRYLGK GVLKAVDHIN STIAPALISS GLSVVEQEKLDNLMLELDGT ENKSKFGANA ILGVSLAVCK AGAAERELPL YRHIAQLAGN SDLILPVPAF NVINGGSHAG NKLAMQEFMI LPVGAESFRDAMRLGAEVYH TLKGVIKDKY GKDATNVGDE GGFAPNILEN SEALELVKEA IDKAGYTEKI VIGMDVAASE FYRDGKYDLD FKSPTDPSRYITGDQLGALY QDFVRDYPVV SIEDPFDQDD WAAWSKFTAN VGIQIVGDDL TVTNPKRIER AVEEKACNCL LLKVNQIGSV TEAIQACKLAQENGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL MRIEEELGDE ARFAGHNFRN PSVL
• Endotoxin: < 1.0 Eu per 1 microgram of protein (determined by LAL method)
• Purity: > 95% by SDS - PAGE
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: ENO2 enolase 2 (gamma, neuronal) [ Homo sapiens (human) ]
• Official Symbol: ENO2
• Synonyms: ENO2; enolase 2 (gamma, neuronal); NSE; EC=4.2.1.11; Gamma-enolase; 2-phospho-D-glycerate hydro-lyase; Neural enolase; Neuron-specific enolase; Enolase 2; neuron specific gamma enolase; 2-phospho-D-glycerate hydrolyase; Neuron-specific enolase; neurone-specific enolase
• Gene ID: 2026
• mRNA Refseq: NM_001975
• Protein Refseq: NP_001966
• MIM: 131360
• UniProt ID: P09104
• Chromosome Location: 12p13
• Pathway: Biosynthesis of amino acids; Carbon metabolism; Gluconeogenesis
• Function: magnesium ion binding; phosphopyruvate hydratase activity