Recombinant Human GOT1, His-tagged

• Cat.No.: GOT1-27058TH
• Product Overview: Recombinant full length Human Aspartate Aminotransferase fused to His Tag at N-terminus; 433aa, 48.4kDa.

Specification

• Description: Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively.GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.
• Protein length: 413 amino acids
• Conjugation: HIS
• Molecular Weight: 48.400kDa inclusive of tags
• Source: E. coli
• Form: Liquid
• Purity: >95% by SDS-PAGE
• Storage buffer: pH: 8.00Constituents:0.32% Tris HCl, 0.03% DTT, 0.58% Sodium chloride, 20% Glycerol
• Storage: Store at 4°C or at -20°C for long term storage.
• Sequences of amino acids: MGSSHHHHHHSSGLVPRGSHMAPPSVFAEVPQAQPVLVFK LTADFREDPDPRKVNLGVGAYRTDDCHPWVLPVVKKVEQK IANDNSLNHEYLPILGLAEFRSCASRLALGDDSPALKEKR VGGVQSLGGTGALRIGADFLARWYNGTNNKNTPVYVSSPT WENHNAVFSAAGFKDIRSYRYWDAEKRGLDLQGFLNDLEN APEFSIVVLHACAHNPTGIDPTPEQWKQIASVMKHRFLFP FFDSAYQGFASGNLERDAWAIRYFVSEGFEFFCAQSFSKN FGLYNERVGNLTVVGKEPESILQVLSQMEKIVRITWSNPP AQGARIVASTLSNPELFEEWTGNVKTMADRILTMRSELRA RLEALKTPGTWNHITDQIGMFSFTGLNPKQVEYLVNEKHI YLLPSGRINVSGLTTKNLDYVATSIHEAVTKIQ
• Sequence Similarities: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

Gene Information

• Gene Name: GOT1 glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1) [ Homo sapiens ]
• Official Symbol: GOT1
• Synonyms: GOT1; glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1); aspartate aminotransferase, cytoplasmic
• Gene ID: 2805
• mRNA Refseq: NM_002079
• Protein Refseq: NP_002070
• MIM: 138180
• Uniprot ID: P17174
• Chromosome Location: 10q24.1-q25.1
• Pathway: Alanine and aspartate metabolism, organism-specific biosystem; Alanine, aspartate and glutamate metabolism, organism-specific biosystem; Alanine, aspartate and glutamate metabolism, conserved biosystem; Amino acid synthesis and interconversion (transamination), organism-specific biosystem; Arginine and proline metabolism, organism-specific biosystem
• Function: L-aspartate:2-oxoglutarate aminotransferase activity; L-aspartate:2-oxoglutarate aminotransferase activity; L-aspartate:2-oxoglutarate aminotransferase activity; L-phenylalanine:2-oxoglutarate aminotransferase activity; carboxylic acid binding