||Recombinant full length mature Human IL5 with His tag at N terminal produced in the endosperm tissue of barley grain ; 131 amino acids with tag, Predicted MWt 15.3 kDa.
||The protein encoded by this gene is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. This cytokine is a main regulator of eosinopoiesis, eosinophil maturation and activation. The elevated production of this cytokine is reported to be related to asthma or hypereosinophilic syndromes. The receptor of this cytokine is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene, together with those for interleukin 4 (IL4), interleukin 13 (IL13), and CSF2, form a cytokine gene cluster on chromosome 5. This cytokine, IL4, and IL13 are found to be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.
||Bioactivity is assayed by measuring the dose dependent effect of Human IL5 on proliferation of TF-1 cells. The EC50 for IL5-27607TH was determined to be 1.51 ng/ml, corresponding to 0.66x 106 U/mg.
||Lyophilised:Reconstitute the lyophilized protein in sterile water to a concentration of no less than 100 μg/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
||>95% by SDS-PAGE
||Preservative: NoneConstituents: PBS, pH 7.2
||The lyophilized protein is stable for a few weeks at room temperature, but best stored at -20°C.
||Belongs to the IL-5 family.