||Recombinant fragment, corresponding to amino acids 328-949 of Human DNA Ligase III with an N terminal His tag. Predicted MWt: 70 kDa.
||This gene is a member of the DNA ligase family. Each member of this family encodes a protein that catalyzes the joining of DNA ends but they each have a distinct role in DNA metabolism. The protein encoded by this gene is involved in excision repair and is located in both the mitochondria and nucleus, with translation initiation from the upstream start codon allowing for transport to the mitochondria and translation initiation from a downstream start codon allowing for transport to the nucleus. Additionally, alternate transcriptional splice variants, encoding different isoforms, have been characterized.
||0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
||KLFSRIFNCNPDDMARDLEQGDVSETIRVFFEQSKSFPPAAKSLLTIQEV DEFLLRLSKL TKEDEQQQALQDIASRCTANDLKCIIRLIKHDLKMNSG AKHVLDALDPNAYEAFKASRNL QDVVERVLHNAQEVEKEPGQRRALSV QASLMTPVQPMLAEACKSVEYAMKKCPNGMFSEI KYDGERVQVHKNGD HFSYFSRSLKPVLPHKVAHFKDYIPQAFPGGHSMILDSEVLLIDNK TG KPLPFGTLGVHKKAAFQDANVCLFVFDCIYFNDVSLMDRPLCERRKFLHD NMVEIPNR IMFSEMKRVTKALDLADMITRVIQEGLEGLVLKDVKGTYE PGKRHWLKVKKDYLNEGAMA DTADLVVLGAFYGQGSKGGMMSIFLMGC YDPGSQKWCTVTKCAGGHDDATLARLQNELDM VKISKDPSKIPSWLKV NKIYYPDFIVPDPKKAAVWEITGAEFSKSEAHTADGISIRFPRC TRIR DDKDWKSATNLPQLKELYQLSKEKADFTVVAGDEGSSTTGGSSEENKGPS GSAVSR KAPSKPSASTKKAEGKLSNSNSKDGNMQTAKPSAMKVGEKLA TKSSPVKVGEKRKAADET LCQTKRRPASEQRGRTVPAGRR
||Upon delivery aliquot and store at -80 centigrade. Avoid freeze / thaw cycles.
||Reconstitute with 105 µl aqua dest.