Recombinant Human LTA, His-tagged

• Cat.No.: LTA-31536TH
• Product Overview: Recombinant full length mature Human TNF beta with His tag, produced in the endosperm tissue of barley grain ; 187 amino acids with tag, Predicted MWt 20.8 kDa.

Specification

• Description: The encoded protein, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. The protein is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. This protein also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis. Genetic variations in this gene are associated with susceptibility to leprosy type 4 and psoriatic arthritis.
• Conjugation: HIS
• Biological activity: Bioactivity:The ED50 is determined by the Cytotoxicity Assay for Human TNF beta for dose-dependent cytolytic effects of serial dilutions of hTNF beta on the fibrosarcoma L-929 cells.The ED50 lies in the range of 1.0-1.6 ng/ml corresponding to 1.0-0.6 x106
• Form: Lyophilised:Reconstitute the lyophilized protein in sterile water to a concentration of no less than 100 μg/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
• Purity: >95% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: PBS, pH 7.2
• Storage: The lyophilized protein is stable for a few weeks at room temperature, but best stored at -20°C.
• Sequence Similarities: Belongs to the tumor necrosis factor family.

Gene Information

• Gene Name: LTA lymphotoxin alpha (TNF superfamily, member 1) [ Homo sapiens ]
• Official Symbol: LTA
• Synonyms: LTA; lymphotoxin alpha (TNF superfamily, member 1); TNFB; lymphotoxin-alpha; LT; TNFSF1
• Gene ID: 4049
• mRNA Refseq: NM_000595
• Protein Refseq: NP_000586
• MIM: 153440
• Uniprot ID: P01374
• Chromosome Location: 6p21.3
• Pathway: Apoptosis, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; HTLV-I infection, organism-specific biosystem; HTLV-I infection, conserved biosystem
• Function: cytokine activity; receptor binding; tumor necrosis factor receptor binding