||Recombinant full length mature Human TNF beta with His tag, produced in the endosperm tissue of barley grain ; 187 amino acids with tag, Predicted MWt 20.8 kDa.
||The encoded protein, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. The protein is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. This protein also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis. Genetic variations in this gene are associated with susceptibility to leprosy type 4 and psoriatic arthritis.
||Bioactivity:The ED50 is determined by the Cytotoxicity Assay for Human TNF beta for dose-dependent cytolytic effects of serial dilutions of hTNF beta on the fibrosarcoma L-929 cells.The ED50 lies in the range of 1.0-1.6 ng/ml corresponding to 1.0-0.6 x106
||Lyophilised:Reconstitute the lyophilized protein in sterile water to a concentration of no less than 100 μg/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
||>95% by SDS-PAGE
||Preservative: NoneConstituents: PBS, pH 7.2
||The lyophilized protein is stable for a few weeks at room temperature, but best stored at -20°C.
||Belongs to the tumor necrosis factor family.