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Recombinant Human MDM2

Cat.No. : MDM2-29009TH
Product Overview : Recombinant full length Human MDM2 Isoform 11 with N terminal proprietary tag; MWt 82kDa.
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  • Gene Information
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Description : This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues.
Protein length : 496 amino acids
Molecular Weight : 82.000kDa inclusive of tags
Source : Baculovirus
Tissue specificity : Ubiquitous. Isoform Mdm2-A, isoform Mdm2-B, isoform Mdm2-C, isoform Mdm2-D, isoform Mdm2-E, isoform Mdm2-F and isoform Mdm2-G are observed in a range of cancers but absent in normal tissues.
Form : Liquid
Storage buffer : Preservative: NoneConstituents: 20% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8.0
Storage : Store at -80°C
Sequence Similarities : Belongs to the MDM2/MDM4 family.Contains 1 RanBP2-type zinc finger.Contains 1 RING-type zinc finger.Contains 1 SWIB domain.
Gene Name : MDM2 Mdm2 p53 binding protein homolog (mouse) [ Homo sapiens ]
Official Symbol : MDM2
Synonyms : MDM2; Mdm2 p53 binding protein homolog (mouse); Mdm2, transformed 3T3 cell double minute 2, p53 binding protein (mouse) , mouse double minute 2, human homolog of; p53 binding protein; E3 ubiquitin-protein ligase Mdm2; HDM2; HDMX; MGC5370;
Gene ID : 4193
mRNA Refseq : NM_002392
Protein Refseq : NP_002383
Uniprot ID : Q00987
Chromosome Location : 12q13-q14
Pathway : AKT phosphorylates targets in the cytosol, organism-specific biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; Apoptosis, organism-specific biosystem; Aurora A signaling, organism-specific biosystem; Bladder cancer, organism-specific biosystem;
Function : enzyme binding; ligase activity; metal ion binding; p53 binding; protein binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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How does MDM2 regulate the tumor suppressor protein p53? 11/20/2022

MDM2 binds directly to p53, inhibiting its transcriptional activity. Additionally, MDM2 ubiquitinates p53, targeting it for proteasomal degradation, thus regulating p53 levels in the cell.

What is the significance of the MDM2-p53 feedback loop in cellular regulation? 11/19/2022

The MDM2-p53 feedback loop is crucial for cellular homeostasis. When DNA damage occurs, p53 levels rise, leading to cell cycle arrest or apoptosis. Elevated p53 can induce MDM2 expression, which in turn downregulates p53, ensuring that p53 activity is transient.

How do amplifications or overexpression of MDM2 contribute to cancer progression? 05/13/2022

Amplifications or overexpression of MDM2 can lead to reduced p53 activity, even in the presence of DNA damage or other stress signals, potentially contributing to uncontrolled cell proliferation and tumorigenesis.

Are there any known inhibitors targeting MDM2, and what are their therapeutic implications? 03/31/2022

Several small molecule inhibitors, like Nutlin-3 and RG7112, target MDM2 to prevent its interaction with p53, thereby stabilizing and activating p53. These inhibitors hold therapeutic potential, especially in tumors with wild-type p53 but overexpressed MDM2.

How does the interaction between MDM2 and other proteins influence cell cycle and apoptosis? 12/20/2021

Besides p53, MDM2 interacts with other proteins, including E2F1 and RB, influencing cell cycle progression and apoptosis. The balance of these interactions can determine cell fate.

What is the primary function of the MDM2 gene and its encoded protein? 02/18/2019

The MDM2 gene encodes a nuclear-localized E3 ubiquitin ligase. Its primary function is to regulate the p53 tumor suppressor protein's stability and activity.

What are the potential therapeutic strategies for targeting MDM2 in cancer treatment? 07/29/2018

Therapeutic strategies targeting MDM2 include the use of MDM2 inhibitors to reactivate p53, combination therapies with DNA-damaging agents, and strategies to disrupt MDM2's interaction with other proteins to modulate cell cycle and apoptosis.

Customer Reviews (3)

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Reviews
12/24/2021

    Validated in multiple assays.

    07/14/2019

      Great for cell culture.

      04/05/2019

        Consistent performance, highly reproducible.

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