Recombinant Human NCBP2, His-tagged

• Cat.No.: NCBP2-29865TH
• Product Overview: Recombinant full length Human NCBP2 (aa 1-156), with N terminal His tag; 176aa inclusive of tag, 20.1 kDa.

Specification

• Description: The product of this gene is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5 cap of nascent pre-mRNA in the nucleoplasm. The encoded protein has an RNP domain commonly found in RNA binding proteins, and contains the cap-binding activity. The CBC promotes pre-mRNA splicing, 3-end processing, RNA nuclear export, and nonsense-mediated mRNA decay. Multiple transcript variants encoding different isoforms have been found for this gene.
• Protein length: 156 amino acids
• Conjugation: HIS
• Molecular Weight: 20.100kDa inclusive of tags
• Source: E. coli
• Form: Liquid
• Purity: >95% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 20% Glycerol, 20mM Tris HCl, 100mM Sodium chloride, 1mM DTT, pH 8
• Storage: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MSGGLLKALR SDSYVELSQY RDQHFRGDNE EQEKLLKKSC TLYVGNLSFY TTEEQIYELF SKSGDIKKII MGLDKMKKTA CGFCFVEYYS RADAENAMRY INGTRLDDRI IRTDWDAGFK EGRQYGRGRS GGQVRDEYRQ DYDAGRGGYG KLAQNQ

Gene Information

• Gene Name: NCBP2 nuclear cap binding protein subunit 2, 20kDa [ Homo sapiens ]
• Official Symbol: NCBP2
• Synonyms: NCBP2; nuclear cap binding protein subunit 2, 20kDa; nuclear cap binding protein subunit 2, 20kD; nuclear cap-binding protein subunit 2; Cbc2; CBP20; NIP1
• Gene ID: 22916
• mRNA Refseq: NM_001042540
• Protein Refseq: NP_001036005
• MIM: 605133
• Uniprot ID: P52298
• Chromosome Location: 3q29
• Pathway: Abortive elongation of HIV-1 transcript in the absence of Tat, organism-specific biosystem; Cap binding complex, organism-specific biosystem; Cleavage of Growing Transcript in the Termination Region, organism-specific biosystem; Formation and Maturation of mRNA Transcript, organism-specific biosystem; Formation of HIV-1 elongation complex containing HIV-1 Tat, organism-specific biosystem
• Function: RNA 7-methylguanosine cap binding; NOT RNA binding; RNA cap binding; nucleotide binding; protein binding