| Description : |
The protein encoded by this gene is a small secreted cysteine-rich protein and a member of the CCN family of regulatory proteins. CNN family proteins associate with the extracellular matrix and play an important role in cardiovascular and skeletal development, fibrosis and cancer development. |
| Source : |
E.coli |
| Species : |
Human |
| Form : |
Lyophilized from a 0.2μm filtered concentrated solution in 20 mM Tris-HCl, pH 8.6, 150 mM NaCl. |
| Bio-activity : |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 1.0 μg/ml, corresponding to a specific activity of > 1000 IU/mg. |
| Molecular Mass : |
Approximately 36.2 kDa, a single non-glycosylated polypeptide chain containing 331 amino acids. |
| Protein length : |
331 |
| AA Sequence : |
MQVAATQRCPPQCPGRCPATPPTCA PGVRAVLDGCSCCLVCARQRGESCS DLEPCDESSGLYCDRSADPSNQTGI CTAVEGDNCVFDGVIYRSGEKFQPS CKFQCTCRDGQIGCVPRCQLDVLLP EPNCPAPRKVEVPGECCEKWICGPD EEDSLGGLTLAAYRPEATLGVEVSD SSVNCIEQTTEWTACSKSCGMGFST RVTNRNRQCEMLKQTRLCMVRPCEQ EPEQPTDKKGKKCLRTKKSLKAIHL QFKNCTSLHTYKPRFCGVCSDGRCC TPHNTKTIQAEFQCSPGQIVKKPVM VIGTCTCHTNCPKNNEAFLQELELK TTRGKM |
| Endotoxin : |
Less than 0.1 EU/μg of rHuNOV as determined by LAL method. |
| Purity : |
>95% by SDS-PAGE and HPLC analysis. |
| Storage : |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
| Reconstitution : |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions. |
