Recombinant Human PARN

• Cat.No.: PARN-30788TH
• Product Overview: Recombinant full length Human PARN with an N terminal proprietary tag; Predicted MWt 96.4 kDa.

Specification

• Description: The protein encoded by this gene is a 3-exoribonuclease, with similarity to the RNase D family of 3-exonucleases. It prefers poly(A) as the substrate, hence, efficiently degrades poly(A) tails of mRNAs. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs. This protein is also involved in silencing of certain maternal mRNAs during oocyte maturation and early embryonic development, as well as in nonsense-mediated decay (NMD) of mRNAs that contain premature stop codons. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Protein length: 639 amino acids
• Molecular Weight: 96.400kDa inclusive of tags
• Source: Wheat germ
• Tissue specificity: Ubiquitous.
• Form: Liquid
• Purity: Proprietary Purification
• Storage buffer: pH: 8.00Constituents:0.3% Glutathione, 0.79% Tris HCl
• Storage: Shipped on dry ice. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
• Sequences of amino acids: MEIIRSNFKSNLHKVYQAIEEADFFAIDGEFSGISDGPSV SALTNGFDTPEERYQKLKKHSMDFLLFQFGLCTFKYDYTD SKYITKSFNFYVFPKPFNRSSPDVKFVCQSSSIDFLASQG FDFNKVFRNGIPYLNQEEERQLREQYDEKRSQANGAGALS YVSPNTSKCPVTIPEDQKKFIDQVVEKIEDLLQSEENKNL DLEPCTGFQRKLIYQTLSWKYPKGIHVETLETEKKERYIV ISKVDEEERKRREQQKHAKEQEELNDAVGFSRVIHAIANS GKLVIGHNMLLDVMHTVHQFYCPLPADLSEFKEMTTCVFP RLLDTKLMASTQPFKDIINNTSLAELEKRLKETPFNPPKV ESAEGFPSYDTASEQLHEAGYDAYITGLCFISMANYLGSF LSPPKIHVSARSKLIEPFFNKLFLMRVMDIPYLNLEGPDL QPKRDHVLHVTFPKEWKTSDLYQLFSAFGNIQISWIDDTS AFVSLSQPEQVKIAVNTSKYAESYRIQTYAEYMGRKQEEK QIKRKWTEDSWKEADSKRLNPQCIPYTLQNHYYRNNSFTA PSTVGKRNLSPSQEEAGLEDGVSGEISDTELEQTDSCAEP LSEGRKKAKKLKRMKKELSPAGSISKNSPATLFEVPDTW
• Sequence Similarities: Belongs to the CAF1 family.Contains 1 R3H domain.

Gene Information

• Gene Name: PARN poly(A)-specific ribonuclease [ Homo sapiens ]
• Official Symbol: PARN
• Synonyms: PARN; poly(A)-specific ribonuclease; poly(A) specific ribonuclease (deadenylation nuclease); poly(A)-specific ribonuclease PARN; DAN; deadenylation nuclease
• Gene ID: 5073
• mRNA Refseq: NM_001134477
• Protein Refseq: NP_001127949
• MIM: 604212
• Uniprot ID: O95453
• Chromosome Location: 16p13
• Pathway: Deadenylation of mRNA, organism-specific biosystem; Deadenylation-dependent mRNA decay, organism-specific biosystem; Destabilization of mRNA by KSRP, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of RNA, organism-specific biosystem
• Function: RNA binding; exonuclease activity; hydrolase activity; mRNA 3-UTR binding; metal ion binding