Recombinant Human PCK1, His-tagged
Cat.No. : | PCK1-29962TH |
Product Overview : | Recombinant full length protein of Human PCK1 fused to His tag at N-terminus, 646 amino acids with a predicted MWt 71.7kDa. |
- Specification
- Gene Information
- Related Products
Description : | This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. Defects in this gene are a cause of cytosolic phosphoenolpyruvate carboxykinase deficiency. A mitochondrial isozyme of the encoded protein also has been characterized. |
Protein length : | 622 amino acids |
Conjugation : | HIS |
Molecular Weight : | 71.700kDa inclusive of tags |
Source : | E. coli |
Tissue specificity : | Major sites of expression are liver, kidney and adipocytes. |
Form : | Liquid |
Purity : | >90% by SDS-PAGE |
Storage buffer : | pH: 8.00Constituents:0.32% Tris HCl, 10% Glycerol, 0.02% DTT, 0.58% Sodium chloride |
Storage : | Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles. |
Sequences of amino acids : | MGSSHHHHHH SSGLVPRGSH MGSHMPPQLQ NGLNLSAKVV QGSLDSLPQA VREFLENNAE LCQPDHIHIC DGSEEENGRL LGQMEEEGIL RRLKKYDNCW LALTDPRDVA RIESKTVIVT QEQRDTVPIP KTGLSQLGRW MSEEDFEKAF NARFPGCMKG RTMYVIPFSM GPLGSPLSKI GIELTDSPYV VASMRIMTRM GTPVLEALGD GEFVKCLHSV GCPLPLQKPL VNNWPCNPEL TLIAHLPDRR EIISFGSGYG GNSLLGKKCF ALRMASRLAK EEGWLAEHML VLGITNPEGE KKYLAAAFPS ACGKTNLAMM NPSLPGWKVE CVGDDIAWMK FDAQGHLRAI NPENGFFGVA PGTSVKTNPN AIKTIQKNTI FTNVAETSDG GVYWEGIDEP LASGVTITSW KNKEWSSEDG EPCAHPNSRF CTPASQCPII DAAWESPEGV PIEGIIFGGR RPAGVPLVYE ALSWQHGVFV GAAMRSEATA AAEHKGKIIM HDPFAMRPFF GYNFGKYLAH WLSMAQHPAA KLPKIFHVNW FRKDKEGKFL WPGFGENSRV LEWMFNRIDG KASTKLTPIG YIPKEDALNL KGLGHINMME LFSISKEFWE KEVEDIEKYL EDQVNADLPC EIEREILALK QRISQM |
Sequence Similarities : | Belongs to the phosphoenolpyruvate carboxykinase [GTP] family. |
Gene Name : | PCK1 phosphoenolpyruvate carboxykinase 1 (soluble) [ Homo sapiens ] |
Official Symbol : | PCK1 |
Synonyms : | PCK1; phosphoenolpyruvate carboxykinase 1 (soluble); phosphoenolpyruvate carboxykinase, cytosolic [GTP]; PEPCK C; |
Gene ID : | 5105 |
mRNA Refseq : | NM_002591 |
Protein Refseq : | NP_002582 |
MIM : | 614168 |
Uniprot ID : | P35558 |
Chromosome Location : | 20q13.31 |
Pathway : | Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem; Adipogenesis, organism-specific biosystem; Citrate cycle (TCA cycle), organism-specific biosystem; Citrate cycle (TCA cycle), conserved biosystem; |
Function : | GDP binding; GTP binding; carboxylic acid binding; lyase activity; magnesium ion binding; |
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◆ Lysates | ||
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Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (6)
Ask a questionRecent studies have found that PCK1 is also involved in the synthesis and oxidation of fatty acids. These processes have an important impact on energy metabolism and cell growth, and also provide new ideas for the treatment of some PCK1-related diseases.
There are still some limitations in the current research on PCK1, such as the lack of in-depth research on its specific mechanism of action and function, and the incomplete understanding of its relationship with diseases. In addition, since PCK1 is a key enzyme in the gluconeogenesis pathway, its regulation may also have some side effects.
PCK1 plays a key role in energy metabolism. By participating in the gluconeogenesis pathway, it maintains a stable supply of glucose in the body, thereby ensuring the body's energy needs in stressful states such as hunger and exercise.
The activity of PCK1 is regulated by a variety of factors, including hormones (such as insulin, glucagon, etc.), nutrients (such as glucose, fatty acids, etc.), and intracellular signal transduction pathways (such as AMPK, mTOR, etc.). These factors regulate the activity of PCK1 by influencing its phosphorylation, acetylation and other modifications.
The expression level of PCK1 can be detected by enzyme-linked immunosorbent assay, immunohistochemistry, real-time PCR and other methods. These methods can detect protein or mRNA levels of PCK1 in tissues or cells to assess changes in its expression in disease.
Therapeutic strategies for PCK1 include inhibiting its expression, modulating its activity, or using gene therapy. For example, some glucose-lowering drugs (such as metformin) reduce blood sugar levels by inhibiting the activity of PCK1 and reducing the output of glucose in the liver. In addition, it is also possible to treat related diseases by regulating the expression level of PCK1.
Customer Reviews (3)
Write a reviewIt has good solubility and can be easily dissolved.
I performed well when doing experiments such as SDS-PAGE, and the results were great.
The synthesis efficiency is high, the yield is high, and the stability is good.
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