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Recombinant Human PCK1, His-tagged

Cat.No. : PCK1-29962TH
Product Overview : Recombinant full length protein of Human PCK1 fused to His tag at N-terminus, 646 amino acids with a predicted MWt 71.7kDa.
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Description : This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. Defects in this gene are a cause of cytosolic phosphoenolpyruvate carboxykinase deficiency. A mitochondrial isozyme of the encoded protein also has been characterized.
Protein length : 622 amino acids
Conjugation : HIS
Molecular Weight : 71.700kDa inclusive of tags
Source : E. coli
Tissue specificity : Major sites of expression are liver, kidney and adipocytes.
Form : Liquid
Purity : >90% by SDS-PAGE
Storage buffer : pH: 8.00Constituents:0.32% Tris HCl, 10% Glycerol, 0.02% DTT, 0.58% Sodium chloride
Storage : Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Sequences of amino acids : MGSSHHHHHH SSGLVPRGSH MGSHMPPQLQ NGLNLSAKVV QGSLDSLPQA VREFLENNAE LCQPDHIHIC DGSEEENGRL LGQMEEEGIL RRLKKYDNCW LALTDPRDVA RIESKTVIVT QEQRDTVPIP KTGLSQLGRW MSEEDFEKAF NARFPGCMKG RTMYVIPFSM GPLGSPLSKI GIELTDSPYV VASMRIMTRM GTPVLEALGD GEFVKCLHSV GCPLPLQKPL VNNWPCNPEL TLIAHLPDRR EIISFGSGYG GNSLLGKKCF ALRMASRLAK EEGWLAEHML VLGITNPEGE KKYLAAAFPS ACGKTNLAMM NPSLPGWKVE CVGDDIAWMK FDAQGHLRAI NPENGFFGVA PGTSVKTNPN AIKTIQKNTI FTNVAETSDG GVYWEGIDEP LASGVTITSW KNKEWSSEDG EPCAHPNSRF CTPASQCPII DAAWESPEGV PIEGIIFGGR RPAGVPLVYE ALSWQHGVFV GAAMRSEATA AAEHKGKIIM HDPFAMRPFF GYNFGKYLAH WLSMAQHPAA KLPKIFHVNW FRKDKEGKFL WPGFGENSRV LEWMFNRIDG KASTKLTPIG YIPKEDALNL KGLGHINMME LFSISKEFWE KEVEDIEKYL EDQVNADLPC EIEREILALK QRISQM
Sequence Similarities : Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.
Gene Name : PCK1 phosphoenolpyruvate carboxykinase 1 (soluble) [ Homo sapiens ]
Official Symbol : PCK1
Synonyms : PCK1; phosphoenolpyruvate carboxykinase 1 (soluble); phosphoenolpyruvate carboxykinase, cytosolic [GTP]; PEPCK C;
Gene ID : 5105
mRNA Refseq : NM_002591
Protein Refseq : NP_002582
MIM : 614168
Uniprot ID : P35558
Chromosome Location : 20q13.31
Pathway : Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem; Adipogenesis, organism-specific biosystem; Citrate cycle (TCA cycle), organism-specific biosystem; Citrate cycle (TCA cycle), conserved biosystem;
Function : GDP binding; GTP binding; carboxylic acid binding; lyase activity; magnesium ion binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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What new mechanisms or functions have been discovered in recent studies on PCK1? 12/07/2022

Recent studies have found that PCK1 is also involved in the synthesis and oxidation of fatty acids. These processes have an important impact on energy metabolism and cell growth, and also provide new ideas for the treatment of some PCK1-related diseases.

Why are studies of PCK1 limited? 12/03/2021

There are still some limitations in the current research on PCK1, such as the lack of in-depth research on its specific mechanism of action and function, and the incomplete understanding of its relationship with diseases. In addition, since PCK1 is a key enzyme in the gluconeogenesis pathway, its regulation may also have some side effects.

What is the role of PCK1 in energy metabolism? 09/15/2021

PCK1 plays a key role in energy metabolism. By participating in the gluconeogenesis pathway, it maintains a stable supply of glucose in the body, thereby ensuring the body's energy needs in stressful states such as hunger and exercise.

How is the activity of PCK1 regulated? 04/11/2021

The activity of PCK1 is regulated by a variety of factors, including hormones (such as insulin, glucagon, etc.), nutrients (such as glucose, fatty acids, etc.), and intracellular signal transduction pathways (such as AMPK, mTOR, etc.). These factors regulate the activity of PCK1 by influencing its phosphorylation, acetylation and other modifications.

How can I detect the expression level of PCK1? 10/18/2020

The expression level of PCK1 can be detected by enzyme-linked immunosorbent assay, immunohistochemistry, real-time PCR and other methods. These methods can detect protein or mRNA levels of PCK1 in tissues or cells to assess changes in its expression in disease.

What are the treatment strategies for PCK1? 08/30/2020

Therapeutic strategies for PCK1 include inhibiting its expression, modulating its activity, or using gene therapy. For example, some glucose-lowering drugs (such as metformin) reduce blood sugar levels by inhibiting the activity of PCK1 and reducing the output of glucose in the liver. In addition, it is also possible to treat related diseases by regulating the expression level of PCK1.

Customer Reviews (3)

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Reviews
05/18/2023

    It has good solubility and can be easily dissolved.

    05/12/2021

      I performed well when doing experiments such as SDS-PAGE, and the results were great.

      04/20/2021

        The synthesis efficiency is high, the yield is high, and the stability is good.

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