Recombinant Human PIK3R1, His-tagged

• Cat.No.: PIK3R1-29588TH
• Product Overview: Recombinant full length Human PI 3 Kinase p85 alpha with N terminal His tag, expressed in a Baculovirus infected Sf9 cell expression system, amino acids 1-724, 86kDa.

Specification

• Description: Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms.
• Conjugation: HIS
• Tissue specificity: Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level).
• Form: Liquid
• Storage buffer: Preservative: 150mM ImidazoleConstituents: 25% Glycerol, 50mM Sodium phosphate, 300mM Sodium chloride, 2mM DTT, 0.1mM PMSF, pH 7.0
• Storage: Shipped on dry ice. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
• Sequence Similarities: Belongs to the PI3K p85 subunit family.Contains 1 Rho-GAP domain.Contains 2 SH2 domains.Contains 1 SH3 domain.

Gene Information

• Gene Name: PIK3R1 phosphoinositide-3-kinase, regulatory subunit 1 (alpha) [ Homo sapiens ]
• Official Symbol: PIK3R1
• Synonyms: PIK3R1; phosphoinositide-3-kinase, regulatory subunit 1 (alpha); phosphatidylinositol 3-kinase regulatory subunit alpha; GRB1; p85; p85 ALPHA
• Gene ID: 5295
• mRNA Refseq: NM_001242466
• Protein Refseq: NP_001229395
• MIM: 171833
• Uniprot ID: P27986
• Chromosome Location: 5q13.1
• Pathway: 3-phosphoinositide biosynthesis, conserved biosystem; Acute myeloid leukemia, organism-specific biosystem; Acute myeloid leukemia, conserved biosystem; Adaptive Immune System, organism-specific biosystem; Aldosterone-regulated sodium reabsorption, organism-specific biosystem
• Function: 1-phosphatidylinositol binding; ErbB-3 class receptor binding; insulin binding; insulin receptor binding; insulin receptor substrate binding