||Recombinant full length Human PIK3CB with N-terminal His tag and recombinant full length Human PIK3R1, untagged, co expressed in a Baculovirus infected Sf9 cell expression system, 124 kDa,and 83kDa respectively.
||Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms.
||Specific Activity: 7.9 pmol/min/μg.A 50 μl kinase reaction is conducted in a buffer containing 10 mM Tris-HCl (pH 7.5), 50 mM NaCl, 5 mM MgCl2, 5 μM ATP, 17 μg Phosphoinositol-4,5-Bisphosphate, and 150 ng enzyme for 90 minutes at room temperature.
||Preservative: NoneConstituents: 30% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8
||Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.