Recombinant Human Human PIK3CB, His-tagged

• Cat.No.: PIK3R1-31767TH
• Product Overview: Recombinant full length Human PIK3CB with N-terminal His tag and recombinant full length Human PIK3R1, untagged, co expressed in a Baculovirus infected Sf9 cell expression system, 124 kDa,and 83kDa respectively.

Specification

• Description: Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms.
• Conjugation: HIS
• Biological activity: Specific Activity: 7.9 pmol/min/μg.A 50 μl kinase reaction is conducted in a buffer containing 10 mM Tris-HCl (pH 7.5), 50 mM NaCl, 5 mM MgCl2, 5 μM ATP, 17 μg Phosphoinositol-4,5-Bisphosphate, and 150 ng enzyme for 90 minutes at room temperature.
• Form: Liquid
• Storage buffer: Preservative: NoneConstituents: 30% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8
• Storage: Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.

Gene Information

• Gene Name: PIK3R1 phosphoinositide-3-kinase, regulatory subunit 1 (alpha) [ Homo sapiens ]
• Official Symbol: PIK3R1
• Synonyms: PIK3R1; phosphoinositide-3-kinase, regulatory subunit 1 (alpha); phosphatidylinositol 3-kinase regulatory subunit alpha; GRB1; p85; p85 ALPHA
• Gene ID: 5295
• mRNA Refseq: NM_001242466
• Protein Refseq: NP_001229395
• MIM: 171833
• Uniprot ID: P27986
• Chromosome Location: 5q13.1
• Pathway: 3-phosphoinositide biosynthesis, conserved biosystem; Acute myeloid leukemia, organism-specific biosystem; Acute myeloid leukemia, conserved biosystem; Adaptive Immune System, organism-specific biosystem; Aldosterone-regulated sodium reabsorption, organism-specific biosystem
• Function: 1-phosphatidylinositol binding; ErbB-3 class receptor binding; insulin binding; insulin receptor binding; insulin receptor substrate binding