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Recombinant Human Superoxide Dismutase

Cat. No.: SOD1-04H
Product Overview: Recombinant Human Superoxide Dismutase produced in E.coli is a homodimer, non-glycosylated polypeptide chain containing 2 x 154 amino acids and having a molecular mass of approximately 31 kDa.
Description: Human Cu/Zn Superoxide Dismutase (SOD) catalyzes the reaction between superoxide anions and hydrogen to yield molecular oxygen and hydrogen peroxide. The enzyme protects the cell against dangerous levels of superoxide.
Purity: >85% by SDS-PAGE and HPLC analyses.
Formulation: Lyophilized from a 0.2μm filtered concentrated (1mg/ml) solution in PBS, pH 7.4.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Specific Activity: Fully biologically active when compared to standard. The potency per mg was tested by Pyrogallic Acid method and was found to be more than 3,000Units/mg.
Endotoxin: LLess than 1EU/μg of rHuSOD as determined by LAL method.
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at <-20oC. Further dilutions should be made in appropriate buffered solutions.
Storage: This lyophilized preparation is stable for several weeks at 2-8oC, but should be kept at -20o for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8oC. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20oC to -70oC. Avoid repeated freeze/thaw cycles.
Gene Name: SOD1 superoxide dismutase 1, soluble [ Homo sapiens ]
Synonyms: SOD1; superoxide dismutase 1, soluble; ALS; SOD; ALS1; IPOA; homodimer; SOD, soluble; indophenoloxidase A; Cu/Zn superoxide dismutase; superoxide dismutase, cystolic; EC 1.15.1.1; Superoxide dismutase [Cu-Zn]
Gene ID: 6647
mRNA Refseq: NM_000454
Protein Refseq: NP_000445
MIM: 147450
UniProt ID: P00441
Chromosome Location: 21q22.1; 21q22.11
Pathway: Amyotrophic lateral sclerosis (ALS); Huntington"s disease; Prion diseases; Hemostasis
Function: antioxidant activity; chaperone binding; copper ion binding; metal ion binding; oxidoreductase activity; protein homodimerization activity; protein phosphatase 2B binding; superoxide dismutase activity; zinc ion binding

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