Recombinant Human PKLR

• Cat.No.: PKLR-30180TH
• Product Overview: Recombinant fragment of Human PKLR with N terminal proprietary tag, 41kDa.

Specification

• Description: The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene.
• Molecular Weight: 41.000kDa
• Source: E. coli
• Form: Lyophilised:Add 100ul of distilled water.
• Storage buffer: pH: 6.80Constituents:1.58% Tris HCl, 4% SDS, 3.09% DTT, 0.2% Bromophenol blue, 20% Sucrose
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
• Sequence Similarities: Belongs to the pyruvate kinase family.

Gene Information

• Gene Name: PKLR pyruvate kinase, liver and RBC [ Homo sapiens ]
• Official Symbol: PKLR
• Synonyms: PKLR; pyruvate kinase, liver and RBC; pyruvate kinase isozymes R/L
• Gene ID: 5313
• mRNA Refseq: NM_000298
• Protein Refseq: NP_000289
• MIM: 609712
• Uniprot ID: P30613
• Chromosome Location: 1q22
• Pathway: ChREBP activates metabolic gene expression, organism-specific biosystem; Developmental Biology, organism-specific biosystem; FOXA2 and FOXA3 transcription factor networks, organism-specific biosystem; Glucose metabolism, organism-specific biosystem; Glycolysis, organism-specific biosystem
• Function: ATP binding; magnesium ion binding; nucleotide binding; potassium ion binding; pyruvate kinase activity