Recombinant Human PTTG1, His-tagged

• Cat.No.: PTTG1-29693TH
• Product Overview: Recombinant full length Human Securin, His-tagged, MW 24.1kDa, aa 1-202.

Specification

• Description: The encoded protein is a homolog of yeast securin proteins, which prevent separins from promoting sister chromatid separation. It is an anaphase-promoting complex (APC) substrate that associates with a separin until activation of the APC. The gene product has transforming activity in vitro and tumorigenic activity in vivo, and the gene is highly expressed in various tumors. The gene product contains 2 PXXP motifs, which are required for its transforming and tumorigenic activities, as well as for its stimulation of basic fibroblast growth factor expression. It also contains a destruction box (D box) that is required for its degradation by the APC. The acidic C-terminal region of the encoded protein can act as a transactivation domain. The gene product is mainly a cytosolic protein, although it partially localizes in the nucleus.
• Conjugation: HIS
• Source: E. coli
• Tissue specificity: Expressed at low level in most tissues, except in adult testis, where it is highly expressed. Overexpressed in many patients suffering from pituitary adenomas, primary epithelial neoplasias, and esophageal cancer.
• Form: Liquid
• Storage buffer: Preservative: NoneConstituents: 10% Glycerol, 0.1M Sodium chloride, 20mM Tris HCl, pH 8
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MATLIYVDKE NGEPGTRVVA KDGLKLGSGP SIKALDGRSQ VSTPRFGKTF DAPPALPKAT RKALGTVNRA TEKSVKTKGP LKQKQPSFSA KKMTEKTVKA KSSVPASDDA YPEIEKFFPF NPLDFESFDL PEEHQIAHLP LSGVPLMILD EERELEKLFQ LGPPSPVKMP SPPWESNLLQ SPSSILSTLD VELPPVCCDI DI
• Sequence Similarities: Belongs to the securin family.

Gene Information

• Gene Name: PTTG1 pituitary tumor-transforming 1 [ Homo sapiens ]
• Official Symbol: PTTG1
• Synonyms: PTTG1; pituitary tumor-transforming 1; TUTR1; securin; EAP1; ESP1 associated protein 1; HPTTG; PTTG; tumor transforming protein 1
• Gene ID: 9232
• mRNA Refseq: NM_004219
• Protein Refseq: NP_004210
• MIM: 604147
• Uniprot ID: O95997
• Chromosome Location: 5q35.1
• Pathway: APC/C-mediated degradation of cell cycle proteins, organism-specific biosystem; APC/C:Cdc20 mediated degradation of Securin, organism-specific biosystem; APC/C:Cdc20 mediated degradation of mitotic proteins, organism-specific biosystem; APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1, organism-specific biosystem; Activation of APC/C and APC/C:Cdc20 mediated degradation of mitotic proteins, organism-specific biosystem
• Function: SH3 domain binding; cysteine-type endopeptidase inhibitor activity; protein binding; sequence-specific DNA binding transcription factor activity