||Lefty proteins are signaling ligands that act in opposition to the NODAL protein. During vertebrate development they regulate the degree of left-right asymmetry by controlling the spatiotemporal influence of the NODAL protein. The role of Lefty1 is to restrict the expression of Lefty2 and Nodal to the left side, and that Lefty2 or Nodal encode a signal for "leftness." Lefty2 serves as a feedback inhibitor to restrict the range of nodal signaling during establishment of the left-right axis.
||By N-terminal sequencing, two peptides corresponding to mouse Lefty-1 propeptide (with Leu 22 at the amino-terminus) and mature Lefty-1 (with a histidine residue at the amino-terminus) are present in the recombinant preparation. In SDS-PAGE under reducing conditions, the propeptide and mature Lefty-1 have apparent molecular masses of 12 kDa and 36 kDa, respectively. The two peptides apparently remained associated after processing and during protein purification.
|State Of Matter:
||>90% by SDS Page and analyzed by silver stain.
||<0.1 ng per 1 µg as determined by the LAL method
|Storage And Stability:
||This lyophilized protein is stable for six to twelve months when stored desiccated at -20°Cto -70°C. After aseptic reconstitution, this protein may be stored at 2°Cto 8°Cfor one month or at -20°Cto -70°Cin a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.