Recombinant Human SIRT1

• Cat.No.: SIRT1-30565TH
• Product Overview: Recombinant fragment: Human Sirtuin 1 , amino acids 193- 741,with an N-terminal enzyme tag.

Specification

• Description: This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants.
• Source: E. coli
• Tissue specificity: Widely expressed.
• Biological activity: Specific Activity: 8 U/ug. One Unit =1 pmol/min, Assay condition: 25 mM Tris/Cl, pH8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 30 uM BPS HDAC substrate, 200 uM NAD+ and 20 ng/ul Sirtuin 1. Incubation condition: 30 min at 30oC.
• Form: Liquid
• Purity: >90% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 0.05% Tween 20, 50mM Tris, 2.7mM Potassium chloride, 138mM Sodium chloride, pH 8.0
• Storage: Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
• Sequence Similarities: Belongs to the sirtuin family.Contains 1 deacetylase sirtuin-type domain.

Gene Information

• Gene Name: SIRT1 sirtuin 1 [ Homo sapiens ]
• Official Symbol: SIRT1
• Synonyms: SIRT1; sirtuin 1; sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae) , sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 1; NAD-dependent deacetylase sirtuin-1; SIR2L1
• Gene ID: 23411
• mRNA Refseq: NM_001142498
• Protein Refseq: NP_001135970
• MIM: 604479
• Uniprot ID: Q96EB6
• Chromosome Location: 10q21
• Pathway: E2F transcription factor network, organism-specific biosystem; Energy Metabolism, organism-specific biosystem; FoxO family signaling, organism-specific biosystem; HIF-2-alpha transcription factor network, organism-specific biosystem; Regulation of Androgen receptor activity, organism-specific biosystem
• Function: HLH domain binding; NOT NAD+ ADP-ribosyltransferase activity; NAD+ binding; NAD-dependent histone deacetylase activity; NAD-dependent histone deacetylase activity (H3-K9 specific)