"SIRT1" Related Products

Expand
Expand
Expand

Recombinant Human SIRT1

Cat.No.: SIRT1-30565TH
Product Overview: Recombinant fragment: Human Sirtuin 1 , amino acids 193- 741,with an N-terminal enzyme tag.
Description: This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants.
Source: E. coli
Tissue specificity: Widely expressed.
Biological activity: Specific Activity: 8 U/ug. One Unit =1 pmol/min, Assay condition: 25 mM Tris/Cl, pH8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 30 uM BPS HDAC substrate, 200 uM NAD+ and 20 ng/ul Sirtuin 1. Incubation condition: 30 min at 30oC.
Form: Liquid
Purity: >90% by SDS-PAGE
Storage buffer: Preservative: NoneConstituents: 0.05% Tween 20, 50mM Tris, 2.7mM Potassium chloride, 138mM Sodium chloride, pH 8.0
Storage: Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
Sequence Similarities: Belongs to the sirtuin family.Contains 1 deacetylase sirtuin-type domain.
Gene Name: SIRT1 sirtuin 1 [ Homo sapiens ]
Official Symbol: SIRT1
Synonyms: SIRT1; sirtuin 1; sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae) , sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 1; NAD-dependent deacetylase sirtuin-1; SIR2L1;
Gene ID: 23411
mRNA Refseq: NM_001142498
Protein Refseq: NP_001135970
MIM: 604479
Uniprot ID: Q96EB6
Chromosome Location: 10q21
Pathway: E2F transcription factor network, organism-specific biosystem; Energy Metabolism, organism-specific biosystem; FoxO family signaling, organism-specific biosystem; HIF-2-alpha transcription factor network, organism-specific biosystem; Regulation of Androgen receptor activity, organism-specific biosystem;
Function: HLH domain binding; NOT NAD+ ADP-ribosyltransferase activity; NAD+ binding; NAD-dependent histone deacetylase activity; NAD-dependent histone deacetylase activity (H3-K9 specific);

Online Inquiry

Note: There will be extra charge for optional service!

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

Optional requirements on this protein    +Expand

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.