Recombinant Human SIRT1, His-tagged

• Cat.No.: SIRT1-31404TH
• Product Overview: Recombinant fragment of Human SIRT1 fused at the N-terminus to a His-tag, 70kDa.

Specification

• Description: This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants.
• Protein length: 554 amino acids
• Conjugation: HIS
• Molecular Weight: 70.000kDa
• Source: E. coli
• Tissue specificity: Widely expressed.
• Biological activity: HDAC activity.
• Form: Liquid
• Purity: >95% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 55mM Tris HCl, 150mM Sodium chloride, pH 8.2
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Sequence Similarities: Belongs to the sirtuin family.Contains 1 deacetylase sirtuin-type domain.

Gene Information

• Gene Name: SIRT1 sirtuin 1 [ Homo sapiens ]
• Official Symbol: SIRT1
• Synonyms: SIRT1; sirtuin 1; sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae) , sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 1; NAD-dependent deacetylase sirtuin-1; SIR2L1
• Gene ID: 23411
• mRNA Refseq: NM_001142498
• Protein Refseq: NP_001135970
• MIM: 604479
• Uniprot ID: Q96EB6
• Chromosome Location: 10q21
• Pathway: E2F transcription factor network, organism-specific biosystem; Energy Metabolism, organism-specific biosystem; FoxO family signaling, organism-specific biosystem; HIF-2-alpha transcription factor network, organism-specific biosystem; Regulation of Androgen receptor activity, organism-specific biosystem
• Function: HLH domain binding; NOT NAD+ ADP-ribosyltransferase activity; NAD+ binding; NAD-dependent histone deacetylase activity; NAD-dependent histone deacetylase activity (H3-K9 specific)