Recombinant Human TNF

• Cat.No.: TNF-30942TH
• Product Overview: Recombinant full length Human TNF alpha expressed in modified human 293 cells; 15-20kDa.

Specification

• Description: This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
• Biological activity: The ED50 of TNF alpha is typically 0.04-0.06 ng/ml as measured in cytotoxicity assay using the TNF alpha susceptible murine WEHI 164 cell line in the presence of actinomycin D.
• Form: Liquid
• Purity: >95% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 10% Trehalose, 1% Human serum albumin, PBS
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Sequences of amino acids: Theoretical sequence:VRSSSRTPSDKPVAHVVANPQAEGQLQWL NRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQ GCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPE GAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFA ESGQVYFGIIAL
• Sequence Similarities: Belongs to the tumor necrosis factor family.

Gene Information

• Gene Name: TNF tumor necrosis factor [ Homo sapiens ]
• Official Symbol: TNF
• Synonyms: TNF; tumor necrosis factor; TNFA, tumor necrosis factor (TNF superfamily, member 2); DIF; TNF superfamily; member 2; TNF alpha; TNFSF2
• Gene ID: 7124
• mRNA Refseq: NM_000594
• Protein Refseq: NP_000585
• MIM: 191160
• Uniprot ID: P01375
• Chromosome Location: 6p21.3
• Pathway: Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem; Adipogenesis, organism-specific biosystem; African trypanosomiasis, organism-specific biosystem; African trypanosomiasis, conserved biosystem
• Function: cytokine activity; identical protein binding; protease binding; protein binding; transcription regulatory region DNA binding