||Recombinant full length soluble Human TNF alpha with His tag produced in the endosperm tissue of barley grain ; 174 amino acids with tag, Predicted MWt 19.6 kDa.
||This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
||Bioactivity:The ED50 is determined by the Cytotoxicity Assay for Human TNF alpha for dose dependent cytolytic effects of serial dilutions of hTNF alpha on the fibrosarcoma L-929 cells. The ED50 for TNF-30945TH was determined to be 40 ng/ml corresponding t
||Lyophilised:Reconstitute the lyophilized protein in sterile phosphate-buffered saline to a concentration of no less than 100 μg/ml. For long term storage of the reconstituted solution it is recommended to add a carrier protein (0.1% HSA or BSA).
||>95% by SDS-PAGE
||Preservative: NoneConstituents: PBS, pH 7.2
||The lyophilized protein is stable for a few weeks at room temperature, but best stored at -20°C.
||Belongs to the tumor necrosis factor family.