Recombinant Human TNF, His-tagged

• Cat.No.: TNF-30945TH
• Product Overview: Recombinant full length soluble Human TNF alpha with His tag produced in the endosperm tissue of barley grain ; 174 amino acids with tag, Predicted MWt 19.6 kDa.

Specification

• Description: This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
• Conjugation: HIS
• Biological activity: Bioactivity:The ED50 is determined by the Cytotoxicity Assay for Human TNF alpha for dose dependent cytolytic effects of serial dilutions of hTNF alpha on the fibrosarcoma L-929 cells. The ED50 for TNF-30945TH was determined to be 40 ng/ml corresponding t
• Form: Lyophilised:Reconstitute the lyophilized protein in sterile phosphate-buffered saline to a concentration of no less than 100 μg/ml. For long term storage of the reconstituted solution it is recommended to add a carrier protein (0.1% HSA or BSA).
• Purity: >95% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: PBS, pH 7.2
• Storage: The lyophilized protein is stable for a few weeks at room temperature, but best stored at -20°C.
• Sequence Similarities: Belongs to the tumor necrosis factor family.

Gene Information

• Gene Name: TNF tumor necrosis factor [ Homo sapiens ]
• Official Symbol: TNF
• Synonyms: TNF; tumor necrosis factor; TNFA, tumor necrosis factor (TNF superfamily, member 2); DIF; TNF superfamily; member 2; TNF alpha; TNFSF2
• Gene ID: 7124
• mRNA Refseq: NM_000594
• Protein Refseq: NP_000585
• MIM: 191160
• Uniprot ID: P01375
• Chromosome Location: 6p21.3
• Pathway: Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem; Adipogenesis, organism-specific biosystem; African trypanosomiasis, organism-specific biosystem; African trypanosomiasis, conserved biosystem
• Function: cytokine activity; identical protein binding; protease binding; protein binding; transcription regulatory region DNA binding